Articles producció científica> Ciències Mèdiques Bàsiques

Evaluation of different conditions and culture media for the recovery of Aeromonas spp. from water and shellfish samples

  • Datos identificativos

    Identificador: PC:1839
    Autores:
    M.J. FiguerasF. Latif-EugenínR. Beaz-Hidalgo
    Resumen:
    DOI: 10.1111/jam.13210 URL: http://onlinelibrary.wiley.com/doi/10.1111/jam.13210/abstract;jsessionid=A8C6238122A177AA995C6BDA1999071C.f02t02 Filiació URV: SI
  • Otros:

    Autor según el artículo: M.J. Figueras; F. Latif-Eugenín; R. Beaz-Hidalgo
    Departamento: Ciències Mèdiques Bàsiques
    Autor/es de la URV: FIGUERAS SALVAT, MARIA JOSEFA; F. Latif-Eugenín; BEAZ HIDALGO, ROXANA
    Palabras clave: Aeromonas shellfish Water
    Resumen: Aims: To perform a comparative study for determining the optimum culture method (direct plating or enrichment) and medium (ampicillin dextrin agar (ADA), starch ampicillin agar (SAA), bile salts irgasan brilliant green modified (BIBG-m)) for recovering Aeromonas species from water and shellfish samples. Methods and Results: By direct culture, Aeromonas was detected in 65% (13/20) of the water samples and in 54·5% (6/11) of the shellfish samples. However, when a pre-enrichment step was included, the number of positive water samples increased to 75% (15/20) and the ones of shellfish to 90·1% (10/11). The enriched culture significantly favoured (P < 0·05) the isolation of Aeromonas allosaccharophila from water, Aeromonas salmonicida from shellfish and Aeromonas caviae from both types of samples. The most specific (P < 0·05) culture medium for detecting Aeromonas from water was ADA. However, no differences were observed in the case of shellfish samples (P > 0·05). Isolation of Aeromonas media from water was favoured (P < 0·05) in the ADA medium, while SAA enhanced (P < 0·05) the isolation of Aer. salmonicida from shellfish. Conclusions: The culture method and medium used influenced the recovery of some Aeromonas species from water and shellfish samples. Significance and Impact of the Study: This fact should be considered in future prevalence studies to avoid overestimating the above mentioned Aeromonas species.
    Grupo de investigación: Unitat de Micologia i Microbiologia Ambiental
    Áreas temáticas: Ciències de la salut Ciencias de la salud Health sciences
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1364-5072
    Identificador del autor: N/D; N/D; N/D
    Fecha de alta del registro: 2016-09-21
    Página final: 891
    Volumen de revista: 121
    Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
    Enlace a la fuente original: https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/jam.13210
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI del artículo: 10.1111/jam.13210
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2016
    Página inicial: 883
    Tipo de publicación: Article Artículo Article
  • Palabras clave:

    Aeromonas
    Aigua--Microbiologia
    Aeromonas
    shellfish
    Water
    Ciències de la salut
    Ciencias de la salud
    Health sciences
    1364-5072
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