Autor según el artículo: M.J. Figueras; F. Latif-Eugenín; R. Beaz-Hidalgo

Departamento: Ciències Mèdiques Bàsiques

Autor/es de la URV: FIGUERAS SALVAT, MARIA JOSEFA; F. Latif-Eugenín; BEAZ HIDALGO, ROXANA

Palabras clave: Aeromonas shellfish Water

Resumen: Aims: To perform a comparative study for determining the optimum culture method (direct plating or enrichment) and medium (ampicillin dextrin agar (ADA), starch ampicillin agar (SAA), bile salts irgasan brilliant green modified (BIBG-m)) for recovering Aeromonas species from water and shellfish samples. Methods and Results: By direct culture, Aeromonas was detected in 65% (13/20) of the water samples and in 54·5% (6/11) of the shellfish samples. However, when a pre-enrichment step was included, the number of positive water samples increased to 75% (15/20) and the ones of shellfish to 90·1% (10/11). The enriched culture significantly favoured (P < 0·05) the isolation of Aeromonas allosaccharophila from water, Aeromonas salmonicida from shellfish and Aeromonas caviae from both types of samples. The most specific (P < 0·05) culture medium for detecting Aeromonas from water was ADA. However, no differences were observed in the case of shellfish samples (P > 0·05). Isolation of Aeromonas media from water was favoured (P < 0·05) in the ADA medium, while SAA enhanced (P < 0·05) the isolation of Aer. salmonicida from shellfish. Conclusions: The culture method and medium used influenced the recovery of some Aeromonas species from water and shellfish samples. Significance and Impact of the Study: This fact should be considered in future prevalence studies to avoid overestimating the above mentioned Aeromonas species.

Grupo de investigación: Unitat de Micologia i Microbiologia Ambiental

Áreas temáticas: Ciències de la salut Ciencias de la salud Health sciences

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1364-5072

Identificador del autor: N/D; N/D; N/D

Fecha de alta del registro: 2016-09-21

Página final: 891

Volumen de revista: 121

Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion

Enlace a la fuente original: https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/jam.13210

DOI del artículo: 10.1111/jam.13210

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2016

Página inicial: 883

Tipo de publicación: Article Artículo Article