Articles producció científica> Bioquímica i Biotecnologia

Determination of dehydrogenase activities involved in D-glucose oxidation in Gluconobacter and Acetobacter strains

  • Datos identificativos

    Identificador: PC:1920
    Handle: https://hdl.handle.net/20.500.11797/PC1920
    Autores:
    María Jesús TorijaFlorencia SainzMinenosuke MatsutaniNaoya KataokaToshiharu YakushiKazunobu MatsushitaAlbert Mas
    Resumen:
    Acetic acid bacteria (AAB) are known for rapid and incomplete oxidation of an extensively variety of alcohols and carbohydrates, resulting in the accumulation of organic acids as the final products. These oxidative fermentations in AAB are catalyzed by PQQ- or FAD- dependent membrane-bound dehydrogenases. In the present study, the enzyme activity of the membrane-bound dehydrogenases [membrane-bound PQQ-glucose dehydrogenase (mGDH), D-gluconate dehydrogenase (GADH) and membrane-bound glycerol dehydrogenase (GLDH)] involved in the oxidation of D-glucose and D-gluconic acid (GA) was determined in six strains of three different species of AAB (three natural and three type strains). Moreover, the effect of these activities on the production of related metabolites [GA, 2-keto-D-gluconic acid (2KGA) and 5-keto-D-gluconic acid (5KGA)] was analyzed. The natural strains belonging to Gluconobacter showed a high mGDH activity and low activity in GADH and GLDH, whereas the Acetobacter malorum strain presented low activity in the three enzymes. Nevertheless, no correlation was observed between the activity of these enzymes and the concentration of the corresponding metabolites. In fact, all the tested strains were able to oxidize D-glucose to GA, being maximal at the late exponential phase of the AAB growth (24 h), which coincided with D-glucose exhaustion and the maximum mGDH activity. Instead, only some of the tested strains were capable of producing 2KGA and/or 5KGA. In the case of Gluconobacter oxydans strains, no 2KGA production was detected which is related to the absence of GADH activity after 24 h, while in the remaining strains, detection of GADH activity after 24 h resulted in a high accumulation of 2KGA. Therefore, it is possible to choose the best strain depending on the

  • Otros:

    Autor según el artículo: María Jesús Torija; Florencia Sainz; Minenosuke Matsutani; Naoya Kataoka; Toshiharu Yakushi; Kazunobu Matsushita; Albert Mas
    Departamento: Bioquímica i Biotecnologia
    Autor/es de la URV: TORIJA MARTÍNEZ, MARÍA JESÚS; Florencia Sainz; Minenosuke Matsutani; Naoya Kataoka; Toshiharu Yakushi; Kazunobu Matsushita; MAS BARON, ALBERTO
    Palabras clave: Keto-D-gluconic acids D-gluconic acid Acetic acid bacteria
    Resumen: Acetic acid bacteria (AAB) are known for rapid and incomplete oxidation of an extensively variety of alcohols and carbohydrates, resulting in the accumulation of organic acids as the final products. These oxidative fermentations in AAB are catalyzed by PQQ- or FAD- dependent membrane-bound dehydrogenases. In the present study, the enzyme activity of the membrane-bound dehydrogenases [membrane-bound PQQ-glucose dehydrogenase (mGDH), D-gluconate dehydrogenase (GADH) and membrane-bound glycerol dehydrogenase (GLDH)] involved in the oxidation of D-glucose and D-gluconic acid (GA) was determined in six strains of three different species of AAB (three natural and three type strains). Moreover, the effect of these activities on the production of related metabolites [GA, 2-keto-D-gluconic acid (2KGA) and 5-keto-D-gluconic acid (5KGA)] was analyzed. The natural strains belonging to Gluconobacter showed a high mGDH activity and low activity in GADH and GLDH, whereas the Acetobacter malorum strain presented low activity in the three enzymes. Nevertheless, no correlation was observed between the activity of these enzymes and the concentration of the corresponding metabolites. In fact, all the tested strains were able to oxidize D-glucose to GA, being maximal at the late exponential phase of the AAB growth (24 h), which coincided with D-glucose exhaustion and the maximum mGDH activity. Instead, only some of the tested strains were capable of producing 2KGA and/or 5KGA. In the case of Gluconobacter oxydans strains, no 2KGA production was detected which is related to the absence of GADH activity after 24 h, while in the remaining strains, detection of GADH activity after 24 h resulted in a high accumulation of 2KGA. Therefore, it is possible to choose the best strain depending on the desired product composition. Moreover, the sequences of these genes were used to construct phylogenetic trees. According to the sequence of gcd, gene coding for mGDH, Acetobacter and Komagataeibacter were phylogenetically more closely related each other than with Gluconobacter.
    Grupo de investigación: Biotecnologia Enològica
    Áreas temáticas: Bioquímica i biotecnologia Bioquímica y tecnología Biochemistry and technology
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1664-302X
    Identificador del autor: 0000-0001-6419-0745; n/a; n/a; n/a; n/a; n/a; 0000-0002-0763-1679
    Fecha de alta del registro: 2016-10-17
    Volumen de revista: 7
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.frontiersin.org/articles/10.3389/fmicb.2016.01358/full
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI del artículo: 10.3389/fmicb.2016.01358
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2016
    Página inicial: Art.num. 1358
    Tipo de publicación: Article Artículo Article

  • Palabras clave:

    Acetobàcter
    Keto-D-gluconic acids
    D-gluconic acid
    Acetic acid bacteria
    Bioquímica i biotecnologia
    Bioquímica y tecnología
    Biochemistry and technology
    1664-302X

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