Autor según el artículo: Rull, A. Garcia, R. Fernandez-Sender, L. Garcia-Heredia, A. Aragones, G. Beltran-Debon, R. Marsillach, J. Alegret, JM. Martin Paredero, V. Mackness, B. Mackness, M. Joven, J. Camps, J

Departamento: Bioquímica i Biotecnologia Medicina i Cirurgia

Autor/es de la URV: Anna Rull; Raúl García; Laura Fernández-Sender; Anabel García Heredia; Gerard Aragonès; Raúl Beltrán-Debón; Judit Marsillach; Josep M. Alegret; Vicente Martín-Paredero; Bharti Mackness; Michael Mackness; , Jorge Joven; Jordi Camps

Resumen: Objective: There are no data on the relationship between serum paraoxonase-3 (PON3) concentration and atherosclerosis in humans. Our aim was to investigate possible associations, using recently developed methods, in patients with peripheral artery disease (PAD) or coronary artery disease (CAD). Methods: We studied 118 PAD and 72 CAD patients and 175 healthy volunteers. Serum PON3 was determined by in-house ELISA using polyclonal antibodies generated against a synthetic peptide with a sequence specific to PON3. Polymorphisms of the PON3 promoter were analyzed by the Iplex Gold MassArrayTM method. Results: There was a significant increase in serum PON3 concentration in both groups of patients with respect to the control group. In PAD patients, we observed significant positive correlations between PON3, insulin levels and HOMA index. These associations were not observed in CAD. There were significant positive associations between serum PON3 and β-2-microglobulin, CCL2 and high-sensitivity C-reactive protein in CAD patients, but not in PAD. We did not find any significant differences in PON3 gene promoter polymorphisms and their haplotypes between patients and controls, indicating that associations were not genetically determined. Conclusion: In both atherosclerotic phenotypes, serum PON3 concentration was increased, but this was associated with decreased insulin sensitivity in PAD and with inflammation in CAD.

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1879-1484

Página final: 551

Volumen de revista: 2

Versión del articulo depositado: info:eu-repo/semantics/submittedVersion

Enlace a la fuente original: http://www.sciencedirect.com/science/article/pii/S0021915011010926

DOI del artículo: 10.1016/j.atherosclerosis.2011.11.021

Entidad: Universitat Rovira i Virgili.

Año de publicación de la revista: 2012

Página inicial: 545