Autor según el artículo: Markéta Svobodová; Miriam Jauset-Rubio; Teresa Mairal; Calum McNeil; Neil Keegan; Ayman Saeed; Mohammad Nooredeen Abbas; Mohammad S. El-Shahawi; Abdulaziz S. Bashammakh; Abdulrahman O. Alyoubi; Ciara K. O´Sullivan
Departamento: Enginyeria Química
Autor/es de la URV: SVOBODOVÁ, MARKÉTA; Miriam Jauset-Rubio; MAIRAL LERGA, TERESA; Calum McNeil; Neil Keegan; Ayman Saeed; Mohammad Nooredeen Abbas; Mohammad S. El-Shahawi; Abdulaziz S. Bashammakh; Abdulrahman O. Alyoubi; O'SULLIVAN, CIARA
Palabras clave: Metal Nanoparticles Nucleic Acid Amplification Techniques
Resumen: Sensitive, specific, rapid, inexpensive and easy-to-use nucleic acid tests for use at the point-of-need are critical for the emerging field of personalised medicine for which companion diagnostics are essential, as well as for application in low resource settings. Here we report on the development of a point-of-care nucleic acid lateral flow test for the direct detection of isothermally amplified DNA. The recombinase polymerase amplification method is modified slightly to use tailed primers, resulting in an amplicon with a duplex flanked by two single stranded DNA tails. This tailed amplicon facilitates detection via hybridisation to a surface immobilised oligonucleotide capture probe and a gold nanoparticle labelled reporter probe. A detection limit of 1 × 10-11 M (190 amol), equivalent to 8.67 × 105 copies of DNA was achieved, with the entire assay, both amplification and detection, being completed in less than 15 minutes at a constant temperature of 37 °C. The use of the tailed primers obviates the need for hapten labelling and consequent use of capture and reporter antibodies, whilst also avoiding the need for any post-amplification processing for the generation of single stranded DNA, thus presenting an assay that can facilely find application at the point of need.
Grupo de investigación: Group of Nanobiotechnology and Bioanalysis
Áreas temáticas: Chemical engineering Ingeniería química Enginyeria química
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 2045-2322
Identificador del autor: n/a; n/a; n/a; n/a; n/a; 0000-0001-5760-5180; 0000-0002-7142-7322; n/a; n/a; n/a; 0000-0002-0965-4655
Fecha de alta del registro: 2016-12-13
Volumen de revista: 6
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://www.nature.com/articles/srep37732
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI del artículo: 10.1038/srep37732
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2016
Página inicial: Art. num. 37732
Tipo de publicación: Article Artículo Article