Autor según el artículo: Maria José Figueras; Arturo Levican; Luis Collado

Departamento: Ciències Mèdiques Bàsiques

Autor/es de la URV: FIGUERAS SALVAT, MARIA JOSEFA; Arturo Levican; Luis Collado

Palabras clave: polymerase chain reaction genetic variability Arcobacter

Resumen: The genus Arcobacter includes species considered emerging food and waterborne pathogens. Despite Arcobacter has been linked to the presence of faecal pollution, few studies have investigated its prevalence in wastewater, and the only isolated species were Arcobacter butzleri and Arcobacter cryaerophilus. This study aimed to establish the prevalence of Arcobacter spp. at a WWTP using in parallel two culturing methods (direct plating and culturing after enrichment) and a direct detection by m-PCR. In addition, the genetic diversity of the isolates was established using the ERIC-PCR genotyping method. Most of the wastewater samples (96.7%) were positive for Arcobacter and a high genetic diversity was observed among the 651 investigated isolates that belonged to 424 different ERIC genotypes. However, only few strains persisted at different dates or sampling points. The use of direct plating in parallel with culturing after enrichment allowed recovering the species A. butzleri, A. cryaerophilus, Arcobacter thereius, Arcobacter defluvii, Arcobacter skirrowii, Arcobacter ellisii, Arcobacter cloacae, and Arcobacter nitrofigilis, most of them isolated for the first time from wastewater. The predominant species was A. butzleri, however, by direct plating predominated A. cryaerophilus. Therefore, the overall predominance of A. butzleri was a bias associated with the use of enrichment.

Grupo de investigación: Unitat de Micologia i Microbiologia Ambiental

Áreas temáticas: Health sciences Ciencias de la salud Ciències de la salut

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 2314-6133

Identificador del autor: N/D; N/D; N/D

Fecha de alta del registro: 2016-12-20

Volumen de revista: 2016

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://www.hindawi.com/journals/bmri/2016/8132058/

DOI del artículo: 10.1155/2016/8132058

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2016

Página inicial: 8132058

Tipo de publicación: Article Artículo Article