Articles producció científica> Bioquímica i Biotecnologia

Anti-inflammatory role of Leptin in glial cells through p38 MAPK pathway inhibition

  • Datos identificativos

    Identificador: PC:2636
    Autores:
    Patraca I, Martínez N, Busquets O, Martí A, Pedrós I, Beas-Zarate C, Marin M, Ettcheto M, Sureda F, Auladell C, Camins A, Folch J.
    Resumen:
    Filiació URV: SI
  • Otros:

    Autor según el artículo: Patraca I, Martínez N, Busquets O, Martí A, Pedrós I, Beas-Zarate C, Marin M, Ettcheto M, Sureda F, Auladell C, Camins A, Folch J.
    Departamento: Bioquímica i Biotecnologia
    Autor/es de la URV: PATRACA FIERRO, JOSÉ IVÁN; MARTINEZ LOPEZ, NOHORA MILENA; Martí, A.; Pedrós, I.; Beas-Zarate, C.; Marin, M.; Ettcheto, M.; Sureda, F.; Auladell, C.; Camins, A.; FOLCH LOPEZ, JAUME
    Palabras clave: Leptin
    Resumen: Background In the present work, we studied the modulatory effect of Leptin (Lep) against pro-inflammatory cytokines, tumour necrosis factor-alpha (TNFα), interleukin 1-beta (IL1β) and interferon-gamma (IFNγ), in primary glial cell cultures. Methods Glial cultures were treated with pro-inflammatory cytokines (TNFα, 20 ng/ml; IL1β, 20 ng/ml; IFNγ 20 ng/ml). Cells were pre-treated with Lep 500 nM, 1 h prior to cytokine treatment. NO released from glial cells was determined using the Griess reaction. Cell viability was determined by the MTT method. Protein expression was determined by western blot. Results Pre-treatment with 500 nM Lep produced an inhibitory effect on inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production after glial cells exposure to pro-inflammatory cytokines. Anti-inflammatory effect can be related to a decrease in P38 MAP Kinase (MAPK) pathway activity. Treatment of glial cell cultures with Lep also reduced the intrinsic apoptotic pathway (cytochrome c release and caspase-3 activation). Conclusions We suggest that Lep would act as an anti-inflammatory factor in glial cells exposed to pro-inflammatory cytokines, exerting its function on p38 MAPK pathway and reducing NO production.
    Grupo de investigación: Farmacobiologia Cel.lular
    Áreas temáticas: Bioquímica i biotecnologia Bioquímica y tecnología Biochemistry and technology
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1734-1140
    Fecha de alta del registro: 2017-03-14
    Página final: 418
    Volumen de revista: 69
    Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
    Enlace a la fuente original: https://www.sciencedirect.com/science/article/abs/pii/S1734114016304273
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI del artículo: 10.1016/j.pharep.2016.12.005
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2017
    Página inicial: 409
    Tipo de publicación: Article Artículo Article
  • Palabras clave:

    Leptina
    Neuròglia
    Citoquines
    Leptin
    Bioquímica i biotecnologia
    Bioquímica y tecnología
    Biochemistry and technology
    1734-1140
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