Articles producció científica> Bioquímica i Biotecnologia

Role of Mitochondrial Retrograde Pathway in Regulating Ethanol-Inducible Filamentous Growth in Yeast

  • Datos identificativos

    Identificador: PC:2804
    Autores:
    Mas, A.González, B.Beltran, G.Cullen, P.J.Torija, M.J.
    Resumen:
    In yeast, ethanol is produced as a by-product of fermentation through glycolysis. Ethanol also stimulates a developmental foraging response called filamentous growth and is thought to act as a quorum-sensing molecule. Ethanol-inducible filamentous growth was examined in a small collection of wine/European strains, which validated ethanol as an inducer of filamentous growth. Wine strains also showed variability in their filamentation responses, which illustrates the striking phenotypic differences that can occur among individuals. Ethanol-inducible filamentous growth in 61278b strains was independent of several of the major filamentation regulatory pathways [including fMAPK, RAS-cAMP, Snf1, Rpd3(L), and Rim101] but required the mitochondrial retrograde (RTG) pathway, an inter-organellar signaling pathway that controls the nuclear response to defects in mitochondrial function. The RTG pathway regulated ethanol-dependent filamentous growth by maintaining flux through the TCA cycle. The ethanol-dependent invasive growth response required the polarisome and transcriptional induction of the cell adhesion molecule Flo11p. Our results validate established stimuli that trigger filamentous growth and show how stimuli can trigger highly specific responses among individuals. Our results also connect an inter-organellar pathway to a quorum sensing response in fungi.
  • Otros:

    Autor según el artículo: Mas, A.; González, B.; Beltran, G.; Cullen, P.J.; Torija, M.J.
    Departamento: Bioquímica i Biotecnologia
    Autor/es de la URV: MAS BARON, ALBERTO; González, B.; BELTRAN CASELLAS, GEMMA; Cullen, P.J.; TORIJA MARTÍNEZ, MARÍA JESÚS
    Palabras clave: filamentous growth pseudohyphal growth quorum sensing
    Resumen: In yeast, ethanol is produced as a by-product of fermentation through glycolysis. Ethanol also stimulates a developmental foraging response called filamentous growth and is thought to act as a quorum-sensing molecule. Ethanol-inducible filamentous growth was examined in a small collection of wine/European strains, which validated ethanol as an inducer of filamentous growth. Wine strains also showed variability in their filamentation responses, which illustrates the striking phenotypic differences that can occur among individuals. Ethanol-inducible filamentous growth in 61278b strains was independent of several of the major filamentation regulatory pathways [including fMAPK, RAS-cAMP, Snf1, Rpd3(L), and Rim101] but required the mitochondrial retrograde (RTG) pathway, an inter-organellar signaling pathway that controls the nuclear response to defects in mitochondrial function. The RTG pathway regulated ethanol-dependent filamentous growth by maintaining flux through the TCA cycle. The ethanol-dependent invasive growth response required the polarisome and transcriptional induction of the cell adhesion molecule Flo11p. Our results validate established stimuli that trigger filamentous growth and show how stimuli can trigger highly specific responses among individuals. Our results also connect an inter-organellar pathway to a quorum sensing response in fungi.
    Grupo de investigación: Biotecnologia Enològica
    Áreas temáticas: Enologia Enología Oenology
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1664-042X
    Identificador del autor: 0000-0002-0763-1679; n/a; 0000-0002-7071-205X; n/a; 0000-0001-6419-0745
    Fecha de alta del registro: 2017-05-18
    Volumen de revista: 8
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: http://journal.frontiersin.org/article/10.3389/fphys.2017.00148/full
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI del artículo: 10.3389/fphys.2017.00148
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2017
    Página inicial: Art.num. 148
    Tipo de publicación: Article Artículo Article
  • Palabras clave:

    Llevats
    Mitocondris
    filamentous growth
    pseudohyphal growth
    quorum sensing
    Enologia
    Enología
    Oenology
    1664-042X
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