Autor según el artículo: Martínez-Micaelo, N.; Beltrán-Debón, R.; Baiges, I.; Faiges, M.; Alegret, J.M.

Departamento: Bioquímica i Biotecnologia Medicina i Cirurgia

Autor/es de la URV: MARTÍNEZ MICAELO, NIEVES BEATRIZ; BELTRÁN DEBÓN, RAÚL ALEJANDRO; BAIGES BLANCO, ISABEL MARIA; Faiges, M.; ALEGRET COLOMÉ, JOSEP MARIA

Palabras clave: Aortic dilation BAV bicuspid aortic valve microRNAs

Resumen: Background: We aimed to determine the circulating miRNA expression profile associated with BAV and aortic dilation to provide diagnostic and prognostic biomarkers for BAV and/or aortic dilation. Methods and results: We applied a miRNome-wide microarray approach using plasma samples (n = 24) from healthy tricuspid aortic valve individuals, BAV patients and BAV patients with aortic dilation to compare and identify the specific miRNAs associated with BAV and aortic dilation. In a second stage, the expression patterns of the miRNA candidates were validated by RT-qPCR in an independent cohort (n = 43). The miRNA microarray data and RT-qPCR analyses revealed that the expression levels of circulating miR-122, miR-130a and miR-486 are significantly influenced by the morphology of the aortic valve (bicuspid/tricuspid) and could be functionally involved in the regulation of TGF-β1 signalling. Furthermore, the expression pattern of miR-718 in the plasma was strongly influenced by dilation of the ascending aorta. miR-718 expression was inversely correlated with the aortic diameter (R = -0.63, p = 3.1 × 10-5) and was an independent predictor of aortic dilation (β = -0.41, p = 0.022). The genes targeted by miR-718 are involved in the regulation of vascular remodelling. Conclusions: We propose that miR-122, miR-130a, miR-486 and miR-718 are new molecular features associated with BAV and aortic dilation principally by the activation of TGF-β1 pathway and vascular remodelling mediated by VEGF signalling pathways.

Grupo de investigación: Grup de Recerca en Nutrigenòmica Unitat de Recerca Biomèdica Quimioinformàtica i Nutrició

Áreas temáticas: Ciències de la salut Ciencias de la salud Health sciences

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1479-5876

Identificador del autor: ; 0000-0001-9691-1906; ; ;

Fecha de alta del registro: 2017-05-18

Volumen de revista: 15

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-017-1176-x

DOI del artículo: https://doi.org/10.1186/s12967-017-1176-x

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2017

Página inicial: 76

Tipo de publicación: Article Artículo Article