Repositori URV

Autor según el artículo: Fernández-Castillejo, Sara; Yuste, Sílvia; Macià, Alba; Ludwig, Izíar; Romero, María-Paz; Catalán, Úrsula; Motilva, Maria-José; Rubió, Laura

Departamento: Medicina i Cirurgia

Autor/es de la URV: FERNÁNDEZ CASTILLEJO, SARA; Yuste, Sílvia; Macià, Alba; Ludwig, Izíar; Romero, María-Paz; CATALÁN SANTOS, ÚRSULA; Motilva, Maria-José; Rubió, Laura

Palabras clave: phenolic metabolites Plasma samples

Resumen: SCOPE: The application of dried blood spot (DBS) cards for the study in human blood of dietary polyphenol bioavailability has been poorly studied. METHODS AND RESULTS: An analytical method based on blood sampling with DBS cards combined with LC-MS/MS has been developed and validated. To test the method validation, the phenolic metabolites are determined in human blood and plasma obtained after an acute intake of a red-fleshed apple snack in ten volunteers. Capillary blood by finger prick is compared to venous blood by venipuncture and whole blood is also compared to their corresponding venous plasma samples. Moreover, the venous plasma results using DBS cards are compared to those obtained by microElution solid phase extraction (µSPE). The main phenolic metabolites detected in blood and plasma samples are phloretin glucuronide, dihydroxyphenylpropionic acid sulphate, (methyl) catechol sulphate, catechol glucuronide, and hydroxyphenyl-γ-valerolactone glucuronide. No significant differences are observed between capillary blood, venous blood, and plasma samples using DBS, and neither between plasma samples analyzed by DBS or µSPE. CONCLUSIONS: Finger-prick blood sampling based on DBS appears to be a suitable alternative to the classic invasive venipuncture for the determination of circulating phenolic metabolites in nutritional postprandial studies.

Grupo de investigación: Unitat de Recerca de Lípids i Arteriosclerosi

Áreas temáticas: Health sciences Ciencias de la salud Ciències de la salut

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1613-4125

Identificador del autor: 0000-0002-8109-9713; ; ; ; ; 0000-0001-8884-9823; ;

Fecha de alta del registro: 2019-03-22

Volumen de revista: 62

Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion

Enlace a la fuente original: https://onlinelibrary.wiley.com/doi/10.1002/mnfr.201800623

URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/

DOI del artículo: 10.1002/mnfr.201800623

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2018

Página inicial: 1800623

Tipo de publicación: Article Artículo Article