Autor según el artículo: Malpique, R. Figueiredo, H. Esteban, Y. Rebuffat, S.A. Hanzu, F.A. Vinaixa, M. Yanes, O. Correig, X. Barceló-Batllori, S. Gasa, R. Kalko, S.G. Gomis, R.
Departamento: Enginyeria Química Enginyeria Mecànica
e-ISSN: 1432-0428
Resumen: Aims/hypothesis: Comprehensive characterisation of the interrelation between the peripancreatic adipose tissue and the pancreatic islets promises novel insights into the mechanisms that regulate beta cell adaptation to obesity. Here, we sought to determine the main pathways and key molecules mediating the crosstalk between these two tissues during adaptation to obesity by the way of an integrated inter-tissue, multi-platform analysis. Methods: Wistar rats were fed a standard or cafeteria diet for 30 days. Transcriptomic variations by diet in islets and peripancreatic adipose tissue were examined through microarray analysis. The secretome from peripancreatic adipose tissue was subjected to a non-targeted metabolomic and proteomic analysis. Gene expression variations in islets were integrated with changes in peripancreatic adipose tissue gene expression and protein and metabolite secretion using an integrated inter-tissue pathway and network analysis. Results: The highest level of data integration, linking genes differentially expressed in both tissues with secretome variations, allowed the identification of significantly enriched canonical pathways, such as the activation of liver/retinoid X receptors, triacylglycerol degradation, and regulation of inflammatory and immune responses, and underscored interaction network hubs, such as cholesterol and the fatty acid binding protein 4, which were unpredicted through single-tissue analysis and have not been previously implicated in the peripancreatic adipose tissue crosstalk with beta cells. Conclusions/interpretation: The integrated analysis reported here allowed the identification of novel mechanisms and key molecules involved in peripancreatic adipose tissue interrelation with beta cells during the development of obesity; this might help the development of novel strategies to prevent type 2 diabetes.
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Palabra clave otro idioma: Data integration metabolomics obesity Pancreatic islets Peripancreatic adipose tissue
ISSN: 0012-186X
Página final: 1231
Volumen de revista: 57
Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
Enlace a la fuente original: https://link.springer.com/article/10.1007%2Fs00125-014-3205-0
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI del artículo: 10.1007/s00125-014-3205-0
Entidad: Universitat Rovira i Virgili.
Año de publicación de la revista: 2014
Página inicial: 1219
Repositori URV