Autor según el artículo: Malandrino MI, Fucho R, Weber M, Calderon-Dominguez M, Mir JF, Valcarcel L, Escoté X, Gómez-Serrano M, Peral B, Salvadó L, Fernández-Veledo S, Casals N, Vázquez-Carrera M, Villarroya F, Vendrell JJ, Serra D, Herrero L.
Departamento: Medicina i Cirurgia Ciències Mèdiques Bàsiques
Autor/es de la URV: Fernandez Veledo, Sonia / Vendrell Ortega, Juan José
Palabras clave: Type 2 diabetes Obesity Macrophages Inflammation Fatty acid oxidation Cpt1 Adipocytes obesity macrophages inflammation fatty acid oxidation cpt1 adipocytes
Resumen: Lipid overload in obesity and type 2 diabetes is associated with adipocyte dysfunction, inflammation, macrophage infiltration, and decreased fatty acid oxidation (FAO). Here, we report that the expression of carnitine palmitoyltransferase 1A (CPT1A), the rate-limiting enzyme in mitochondrial FAO, is higher in human adipose tissue macrophages than in adipocytes and that it is differentially expressed in visceral vs. subcutaneous adipose tissue in both an obese and a type 2 diabetes cohort. These observations led us to further investigate the potential role of CPT1A in adipocytes and macrophages. We expressed CPT1AM, a permanently active mutant form of CPT1A, in 3T3-L1 CARΔ1 adipocytes and RAW 264.7 macrophages through adenoviral infection. Enhanced FAO in palmitate-incubated adipocytes and macrophages reduced triglyceride content and inflammation, improved insulin sensitivity in adipocytes, and reduced endoplasmic reticulum stress and ROS damage in macrophages. We conclude that increasing FAO in adipocytes and macrophages improves palmitate-induced derangements. This indicates that enhancing FAO in metabolically relevant cells such as adipocytes and macrophages may be a promising strategy for the treatment of chronic inflammatory pathologies such as obesity and type 2 diabetes.Copyright © 2015 the American Physiological Society.
Áreas temáticas: Physiology (medical) Physiology Odontología Nutrição Medicine (all) Medicina ii Medicina i General medicine Farmacia Endocrinology, diabetes and metabolism Endocrinology & metabolism Educação física Ciências biológicas ii Ciências biológicas i Biotecnología
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 15221555
Direcció de correo del autor: sonia.fernandez@urv.cat jvortega@iispv.cat
Identificador del autor: 0000-0003-2906-3788 0000-0002-6994-6115
Fecha de alta del registro: 2024-11-09
Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
Enlace a la fuente original: https://journals.physiology.org/doi/full/10.1152/ajpendo.00362.2014
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: American Journal Of Physiology-Endocrinology And Metabolism. 308 (9): E756-E769
Referencia de l'ítem segons les normes APA: Malandrino MI, Fucho R, Weber M, Calderon-Dominguez M, Mir JF, Valcarcel L, Escoté X, Gómez-Serrano M, Peral B, Salvadó L, Fernández-Veledo S, Casals (2015). Enhanced fatty acid oxidation in adipocytes and macrophages reduces lipid-induced triglyceride accumulation and inflammation. American Journal Of Physiology-Endocrinology And Metabolism, 308(9), E756-E769. DOI: 10.1152/ajpendo.00362.2014
DOI del artículo: 10.1152/ajpendo.00362.2014
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2015
Tipo de publicación: Journal Publications