Autor según el artículo: Malandrino MI, Fucho R, Weber M, Calderon-Dominguez M, Mir JF, Valcarcel L, Escoté X, Gómez-Serrano M, Peral B, Salvadó L, Fernández-Veledo S, Casals N, Vázquez-Carrera M, Villarroya F, Vendrell JJ, Serra D, Herrero L.

Departamento: Medicina i Cirurgia Ciències Mèdiques Bàsiques

Autor/es de la URV: Fernandez Veledo, Sonia / Vendrell Ortega, Juan José

Palabras clave: Type 2 diabetes Obesity Macrophages Inflammation Fatty acid oxidation Cpt1 Adipocytes obesity macrophages inflammation fatty acid oxidation cpt1 adipocytes

Resumen: Lipid overload in obesity and type 2 diabetes is associated with adipocyte dysfunction, inflammation, macrophage infiltration, and decreased fatty acid oxidation (FAO). Here, we report that the expression of carnitine palmitoyltransferase 1A (CPT1A), the rate-limiting enzyme in mitochondrial FAO, is higher in human adipose tissue macrophages than in adipocytes and that it is differentially expressed in visceral vs. subcutaneous adipose tissue in both an obese and a type 2 diabetes cohort. These observations led us to further investigate the potential role of CPT1A in adipocytes and macrophages. We expressed CPT1AM, a permanently active mutant form of CPT1A, in 3T3-L1 CARΔ1 adipocytes and RAW 264.7 macrophages through adenoviral infection. Enhanced FAO in palmitate-incubated adipocytes and macrophages reduced triglyceride content and inflammation, improved insulin sensitivity in adipocytes, and reduced endoplasmic reticulum stress and ROS damage in macrophages. We conclude that increasing FAO in adipocytes and macrophages improves palmitate-induced derangements. This indicates that enhancing FAO in metabolically relevant cells such as adipocytes and macrophages may be a promising strategy for the treatment of chronic inflammatory pathologies such as obesity and type 2 diabetes.Copyright © 2015 the American Physiological Society.

Áreas temáticas: Physiology (medical) Physiology Odontología Nutrição Medicine (all) Medicina ii Medicina i General medicine Farmacia Endocrinology, diabetes and metabolism Endocrinology & metabolism Educação física Ciências biológicas ii Ciências biológicas i Biotecnología

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 15221555

Direcció de correo del autor: sonia.fernandez@urv.cat juanjose.vendrell@urv.cat

Identificador del autor: 0000-0003-2906-3788 0000-0002-6994-6115

Fecha de alta del registro: 2023-07-31

Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion

Enlace a la fuente original: https://journals.physiology.org/doi/full/10.1152/ajpendo.00362.2014

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: American Journal Of Physiology-Endocrinology And Metabolism. 308 (9): E756-E769

Referencia de l'ítem segons les normes APA: Malandrino MI, Fucho R, Weber M, Calderon-Dominguez M, Mir JF, Valcarcel L, Escoté X, Gómez-Serrano M, Peral B, Salvadó L, Fernández-Veledo S, Casals (2015). Enhanced fatty acid oxidation in adipocytes and macrophages reduces lipid-induced triglyceride accumulation and inflammation. American Journal Of Physiology-Endocrinology And Metabolism, 308(9), E756-E769. DOI: 10.1152/ajpendo.00362.2014

DOI del artículo: 10.1152/ajpendo.00362.2014

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2015

Tipo de publicación: Journal Publications