Articles producció científica> Enginyeria Química

Cell-based assay coupled with chromatographic fractioning: A strategy for marine toxins detection in natural samples

  • Datos identificativos

    Identificador: imarina:3190404
  • Autores:

    Diogene, J.
    Gimenez, G.
    de la Iglesia, P.
    Canete, E.
    Caillaud, A.
  • Otros:

    Autor según el artículo: Diogene, J. Gimenez, G. de la Iglesia, P. Canete, E. Caillaud, A.
    Departamento: REVISAR - Enginyeria Química
    Autor/es de la URV: GIMÉNEZ PAPIOL, GEMMA
    Palabras clave: SHELLFISH Neuroblastoma Microalgae Marine toxins Gambierdiscus Fractioning Fish Cytotoxicity Cell-Based Assay
    Resumen: Cell-based assays (CBA) have been proposed for the evaluation of toxicity caused by marine toxins in natural samples (fish, shellfish and microalgae). However, their application has been hindered due to the interferences present in biological matrices that may cause cellular response and interfere in toxicity evaluation. This work reviews in an extensive introduction the use of CBA for toxicity evaluation of marine toxins. Afterwards, the coupling of chromatographic fractioning with neuroblastoma Neuro-2a CBA is presented to enhance the applicability of CBA for complex matrices. Examples of application are provided for mussel samples (Mytilus galloprovincialis) and microalgae (Gambierdiscus sp.), and the results demonstrated the great potential of the combined strategy for reliable toxicological evaluation without ethical concern. Fractioning of an equivalent of 72 mg eq mL(-1) of mussel sample allowed the identification of non-toxic and toxic fractions whereas only 2.5mg eq mL(-1) of non-purified mussel sample was responsible for 20% of cell mortality. Furthermore, the application of CBA allowed selectively distinguishing between ciguatoxin-like and other unspecific toxicity in Gambierdiscus sp. extract.
    Áreas temáticas: Toxicology Medicine (Miscellaneous)
    Direcció de correo del autor: gemma.gimenez@urv.cat
    ISSN: 08872333
    Fecha de alta del registro: 2020-01-29
    Referencia al articulo segun fuente origial: TOXICOLOGY IN VITRO. (ISSN/ISBN: 08872333). 23(8): 1591-1596
    Referencia de l'ítem segons les normes APA: Caillaud, A.; Canete, E.; de la Iglesia, P.; Gimenez, G.; Diogene, J. (2009). Cell-based assay coupled with chromatographic fractioning: A strategy for marine toxins detection in natural samples. TOXICOLOGY IN VITRO, 23(8), 1591-1596. DOI: 10.1016/j.tiv.2009.08.013
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI del artículo: 10.1016/j.tiv.2009.08.013
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2009
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Medicine (Miscellaneous),Toxicology,Transplantation
    SHELLFISH
    Neuroblastoma
    Microalgae
    Marine toxins
    Gambierdiscus
    Fractioning
    Fish
    Cytotoxicity
    Cell-Based Assay
    Toxicology
    Medicine (Miscellaneous)
    08872333
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