Articles producció científica> Medicina i Cirurgia

The impact of polyphenols on chondrocyte growth and survival: a preliminary report

  • Datos identificativos

    Identificador: imarina:3646798
    Autores:
    Fernández-Arroyo S, Huete-Toral F, Pérez de Lara MJ, de la Luz Cádiz-Gurrea M, Legeai-Mallet L, Micol V, Segura-Carretero A, Joven J, Pintor J.
    Resumen:
    Background: Imbalances in the functional binding of fibroblast growth factors (FGFs) to their receptors (FGFRs) have consequences for cell proliferation and differentiation that in chondrocytes may lead to degraded cartilage. The toxic, proinflammatory, and oxidative response of cytokines and FGFs can be mitigated by dietary polyphenols. Objective: We explored the possible effects of polyphenols in the management of osteoarticular diseases using a model based on the transduction of a mutated human FGFR3 (G380R) in murine chondrocytes. This mutation is present in most cases of skeletal dysplasia and is responsible for the overexpression of FGFR3 that, in the presence of its ligand, FGF9, results in toxic effects leading to altered cellular growth. Design: Different combinations of dietary polyphenols derived from plant extracts were assayed in FGFR3 (G380R) mutated murine chondrocytes, exploring cell survival, chloride efflux, extracellular matrix (ECM) generation, and grade of activation of mitogen-activated protein kinases. Results: Bioactive compounds from Hibiscus sabdariffa reversed the toxic effects of FGF9 and restored normal growth, suggesting a probable translation to clinical requests in humans. Indeed, these compounds activated the intracellular chloride efflux, increased ECM generation, and stimulated cell proliferation. The inhibition of mitogen-activated protein kinase phosphorylation was interpreted as the main mechanism governing these beneficial effects. Conclusions: These findings support the rationale behind the encouragement of the development of drugs that repress the overexpression of FGFRs and suggest the dietary incorporation of supplementary nutrients in the management of degraded cartilage.
  • Otros:

    Autor según el artículo: Fernández-Arroyo S, Huete-Toral F, Pérez de Lara MJ, de la Luz Cádiz-Gurrea M, Legeai-Mallet L, Micol V, Segura-Carretero A, Joven J, Pintor J.
    Departamento: Medicina i Cirurgia
    Autor/es de la URV: FERNANDEZ ARROYO, SALVADOR / Joven Maried, Jorge
    Palabras clave: Signaling pathways Polyphenols Osteoarthritis Nutrition Hibiscus sabdariffa Fibroblast growth factor Chondrodysplasia polyphenols osteoarthritis nutrition hibiscus sabdariffa fibroblast growth factor chondrodysplasia
    Resumen: Background: Imbalances in the functional binding of fibroblast growth factors (FGFs) to their receptors (FGFRs) have consequences for cell proliferation and differentiation that in chondrocytes may lead to degraded cartilage. The toxic, proinflammatory, and oxidative response of cytokines and FGFs can be mitigated by dietary polyphenols. Objective: We explored the possible effects of polyphenols in the management of osteoarticular diseases using a model based on the transduction of a mutated human FGFR3 (G380R) in murine chondrocytes. This mutation is present in most cases of skeletal dysplasia and is responsible for the overexpression of FGFR3 that, in the presence of its ligand, FGF9, results in toxic effects leading to altered cellular growth. Design: Different combinations of dietary polyphenols derived from plant extracts were assayed in FGFR3 (G380R) mutated murine chondrocytes, exploring cell survival, chloride efflux, extracellular matrix (ECM) generation, and grade of activation of mitogen-activated protein kinases. Results: Bioactive compounds from Hibiscus sabdariffa reversed the toxic effects of FGF9 and restored normal growth, suggesting a probable translation to clinical requests in humans. Indeed, these compounds activated the intracellular chloride efflux, increased ECM generation, and stimulated cell proliferation. The inhibition of mitogen-activated protein kinase phosphorylation was interpreted as the main mechanism governing these beneficial effects. Conclusions: These findings support the rationale behind the encouragement of the development of drugs that repress the overexpression of FGFRs and suggest the dietary incorporation of supplementary nutrients in the management of degraded cartilage.
    Áreas temáticas: Saúde coletiva Public health, environmental and occupational health Nutrition and dietetics Nutrition & dietetics Nutrição Medicina veterinaria Medicina ii Medicina i Food science & technology Food science Farmacia Educação física Ciências agrárias i Ciência de alimentos
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: jorge.joven@urv.cat
    Identificador del autor: 0000-0003-2749-4541
    Fecha de alta del registro: 2024-09-07
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.tandfonline.com/doi/full/10.3402/fnr.v59.29311?scroll=top&needAccess=true
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Food & Nutrition Research. 59 (29311): 29311-
    Referencia de l'ítem segons les normes APA: Fernández-Arroyo S, Huete-Toral F, Pérez de Lara MJ, de la Luz Cádiz-Gurrea M, Legeai-Mallet L, Micol V, Segura-Carretero A, Joven J, Pintor J. (2015). The impact of polyphenols on chondrocyte growth and survival: a preliminary report. Food & Nutrition Research, 59(29311), 29311-. DOI: 10.3402/fnr.v59.29311
    DOI del artículo: 10.3402/fnr.v59.29311
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2015
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Food Science,Food Science & Technology,Nutrition & Dietetics,Nutrition and Dietetics,Public Health, Environmental and Occupational Health
    Signaling pathways
    Polyphenols
    Osteoarthritis
    Nutrition
    Hibiscus sabdariffa
    Fibroblast growth factor
    Chondrodysplasia
    polyphenols
    osteoarthritis
    nutrition
    hibiscus sabdariffa
    fibroblast growth factor
    chondrodysplasia
    Saúde coletiva
    Public health, environmental and occupational health
    Nutrition and dietetics
    Nutrition & dietetics
    Nutrição
    Medicina veterinaria
    Medicina ii
    Medicina i
    Food science & technology
    Food science
    Farmacia
    Educação física
    Ciências agrárias i
    Ciência de alimentos
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