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Engineering the missing Link in Biosynthetic (-)-Menthol Production: Bacterial Isopulegone Isomerase

  • Datos identificativos

    Identificador: imarina:3715456
    Autores:
    Currin ADunstan MJohannissen LHollywood KVinaixa MJervis ASwainston NRattray NGardiner JKell DTakano EToogood HScrutton N
    Resumen:
    The realization of a synthetic biology approach to microbial (1R,2S,5R)-(-)-menthol (1) production relies on the identification of a gene encoding an isopulegone isomerase (IPGI), the only enzyme in the Mentha piperita biosynthetic pathway as yet unidentified. We demonstrate that ?5-3-ketosteroid isomerase (KSI) from Pseudomonas putida can act as an IPGI, producing (R)-(+)-pulegone ((R)-2) from (+)-cis-isopulegone (3). Using a robotics-driven semirational design strategy, we identified a key KSI variant encoding four active site mutations, which confer a 4.3-fold increase in activity over the wild-type enzyme. This was assisted by the generation of crystal structures of four KSI variants, combined with molecular modeling of 3 binding to identify key active site residue targets. The KSI variant was demonstrated to function efficiently within cascade biocatalytic reactions with downstream Mentha enzymes pulegone reductase and (-)-menthone:(-)-menthol reductase to generate 1 from 3. This study introduces the use of a recombinant IPGI, engineered to function efficiently within a biosynthetic pathway for the production of 1 in microorganisms.
  • Otros:

    Autor según el artículo: Currin A; Dunstan M; Johannissen L; Hollywood K; Vinaixa M; Jervis A; Swainston N; Rattray N; Gardiner J; Kell D; Takano E; Toogood H; Scrutton N
    Departamento: Enginyeria Electrònica, Elèctrica i Automàtica
    Autor/es de la URV: Vinaixa Crevillent, Maria
    Palabras clave: Robotics Ketosteroid isomerase Isopulegone isomerase Enzyme engineering Biosynthetic (-)-menthol production
    Resumen: The realization of a synthetic biology approach to microbial (1R,2S,5R)-(-)-menthol (1) production relies on the identification of a gene encoding an isopulegone isomerase (IPGI), the only enzyme in the Mentha piperita biosynthetic pathway as yet unidentified. We demonstrate that ?5-3-ketosteroid isomerase (KSI) from Pseudomonas putida can act as an IPGI, producing (R)-(+)-pulegone ((R)-2) from (+)-cis-isopulegone (3). Using a robotics-driven semirational design strategy, we identified a key KSI variant encoding four active site mutations, which confer a 4.3-fold increase in activity over the wild-type enzyme. This was assisted by the generation of crystal structures of four KSI variants, combined with molecular modeling of 3 binding to identify key active site residue targets. The KSI variant was demonstrated to function efficiently within cascade biocatalytic reactions with downstream Mentha enzymes pulegone reductase and (-)-menthone:(-)-menthol reductase to generate 1 from 3. This study introduces the use of a recombinant IPGI, engineered to function efficiently within a biosynthetic pathway for the production of 1 in microorganisms.
    Áreas temáticas: Química Materiais Interdisciplinar General chemistry Engenharias ii Ciências agrárias i Chemistry, physical Chemistry (miscellaneous) Chemistry (all) Catalysis Astronomia / física
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 21555435
    Direcció de correo del autor: maria.vinaixa@urv.cat maria.vinaixa@urv.cat
    Identificador del autor: 0000-0001-9804-0171 0000-0001-9804-0171
    Fecha de alta del registro: 2024-09-07
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://pubs.acs.org/doi/abs/10.1021/acscatal.7b04115
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Acs Catalysis. 8 (3): 2012-2020
    Referencia de l'ítem segons les normes APA: Currin A; Dunstan M; Johannissen L; Hollywood K; Vinaixa M; Jervis A; Swainston N; Rattray N; Gardiner J; Kell D; Takano E; Toogood H; Scrutton N (2018). Engineering the missing Link in Biosynthetic (-)-Menthol Production: Bacterial Isopulegone Isomerase. Acs Catalysis, 8(3), 2012-2020. DOI: 10.1021/acscatal.7b04115
    DOI del artículo: 10.1021/acscatal.7b04115
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2018
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Catalysis,Chemistry (Miscellaneous),Chemistry, Physical
    Robotics
    Ketosteroid isomerase
    Isopulegone isomerase
    Enzyme engineering
    Biosynthetic (-)-menthol production
    Química
    Materiais
    Interdisciplinar
    General chemistry
    Engenharias ii
    Ciências agrárias i
    Chemistry, physical
    Chemistry (miscellaneous)
    Chemistry (all)
    Catalysis
    Astronomia / física
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