Autor según el artículo: Sanchez-Soberon, Francisco; Cuykx, Matthias; Serra, Noemi; Linares, Victoria; Belles, Montserrat; Covaci, Adrian; Schuhmacher, Marta
Departamento: Ciències Mèdiques Bàsiques Enginyeria Química
Autor/es de la URV: Bellés Mateu, Montserrat / Linares Vidal, M. Victoria / SÁNCHEZ SOBERÓN, FRANCISCO / Schuhmacher Ansuategui, Marta
Palabras clave: Particulate matter Metabolomics Indoor sampling Hp-lc/ms Cytotoxicity A549 metabolomics indoor sampling hp-lc/ms cytotoxicity a549
Resumen: In this pilot study three fractions of particulate matter (PM0.25, PM2.5-0.25, and PM10-2.5) were collected in three environments (classroom, home, and outdoors) in a village located nearby an industrial complex. Time-activity pattern of 20 students attending the classroom was obtained, and the dose of particles reaching the children's lungs under actual environmental conditions (i.e. real dose) was calculated via dosimetry model. The highest PM concentrations were reached in the classroom. Simulations showed that heavy intensity outdoor activities played a major role in PM deposition, especially in the upper part of the respiratory tract. The mass of PM10-2.5 reaching the alveoli was minor, while PM2.5-0.25 and PM0.25 apportion for most of the PM mass retained in the lungs. Consequently, PM2.5-0.25 and PM0.25 were the only fractions used in two subsequent toxicity assays onto alveolar cells (A549). First, a cytotoxicity dose-response assay was performed, and doses corresponding to 5% mortality (LC5) were estimated. Afterwards, two LC-MS metabolomic assays were conducted: one applying LC5, and another applying real dose. A lower estimated LC5 value was obtained for PM0.25 than PM2.5-0.25 (8.08 and 73.7 ng/mL respectively). The number of altered features after LC5 exposure was similar for both fractions (39 and 38 for PM0.25 and PM2.5-0.25 respectively), while after real dose exposure these numbers differed (10 and 5 for PM0.25 and PM2.5-0.25 respectively). The most metabolic changes were related to membrane and lung surfactant lipids. This study highlights the capacity of PM to alter metabolic profile of lung cells at conventional environmental levels.
Áreas temáticas: Zootecnia / recursos pesqueiros Saúde coletiva Química Public health, environmental and occupational health Pollution Odontología Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina ii Medicina i Materiais Interdisciplinar Health, toxicology and mutagenesis Geociências General chemistry Farmacia Environmental sciences Environmental engineering Environmental chemistry Ensino Engenharias iii Engenharias ii Engenharias i Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry (miscellaneous) Chemistry (all) Biotecnología Biodiversidade Astronomia / física
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 00456535
Direcció de correo del autor: mvictoria.linares@urv.cat montserrat.belles@urv.cat marta.schuhmacher@urv.cat
Identificador del autor: 0000-0003-4170-4197 0000-0002-9282-2989 0000-0003-4381-2490
Fecha de publicacion del artículo: 2018/10/31
Fecha de alta del registro: 2024-10-12
Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
Enlace a la fuente original: https://www.sciencedirect.com/science/article/abs/pii/S0045653518311408?via%3Dihub
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Chemosphere. 209 137-146
Referencia de l'ítem segons les normes APA: Sanchez-Soberon, Francisco; Cuykx, Matthias; Serra, Noemi; Linares, Victoria; Belles, Montserrat; Covaci, Adrian; Schuhmacher, Marta (2018). In-vitro metabolomics to evaluate toxicity of particulate matter under environmentally realistic conditions. Chemosphere, 209(), 137-146. DOI: 10.1016/j.chemosphere.2018.06.065
DOI del artículo: 10.1016/j.chemosphere.2018.06.065
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2018
Tipo de publicación: Journal Publications