Articles producció científica> Enginyeria Electrònica, Elèctrica i Automàtica

Metabolic Profiling in Formalin-Fixed and Paraffin-Embedded Prostate Cancer Tissues

  • Datos identificativos

    Identificador: imarina:5130959
    Autores:
    Cacciatore SZadra GBango CPenney KTyekucheva SYanes OLoda M
    Resumen:
    Metabolite profiling has significantly contributed to a deeper understanding of the biochemical metabolic networks and pathways in cancer cells. Metabolomics-based biomarker discovery would greatly benefit from the ability to interrogate retrospective annotated clinical specimens archived as formalin-fixed, paraffinembedded (FFPE) material. Mass spectrometry-based metabolomic analysis was performed in matched frozen and FFPE human prostate cancers as well as isogenic prostate cancer cell lines. A total of 352 and 460 metabolites were profiled in human tissues and cell lines, respectively. Classes and physical-chemical characteristics of the metabolites preserved in FFPE material were characterized and related to their preservation or loss following fixation and embedding. Metabolite classes were differentially preserved in archival FFPE tissues, regardless of the age of the block, compared with matched frozen specimen, ranging from maximal preservation of fatty acids (78%) to loss of the majority of peptides and steroids. Generally, FFPE samples showed a decrease of metabolites with functional groups, such as carboxamide. As an adjunct technique, metabolic profiles were also obtained in situ from FFPE tissue sections where metabolites were extracted in a manner that preserves tissue architecture. Despite the fact that selected metabolites were not retained after processing, global metabolic profiles obtained from FFPE can be used to predict biologic states and study biologic pathways. These results pave the way for metabolomics-based biomarker discovery/validation utilizing retrospective and clinically annotated FFPE collections.
  • Otros:

    Autor según el artículo: Cacciatore S; Zadra G; Bango C; Penney K; Tyekucheva S; Yanes O; Loda M
    Departamento: Enginyeria Electrònica, Elèctrica i Automàtica
    Autor/es de la URV: Yanes Torrado, Óscar
    Resumen: Metabolite profiling has significantly contributed to a deeper understanding of the biochemical metabolic networks and pathways in cancer cells. Metabolomics-based biomarker discovery would greatly benefit from the ability to interrogate retrospective annotated clinical specimens archived as formalin-fixed, paraffinembedded (FFPE) material. Mass spectrometry-based metabolomic analysis was performed in matched frozen and FFPE human prostate cancers as well as isogenic prostate cancer cell lines. A total of 352 and 460 metabolites were profiled in human tissues and cell lines, respectively. Classes and physical-chemical characteristics of the metabolites preserved in FFPE material were characterized and related to their preservation or loss following fixation and embedding. Metabolite classes were differentially preserved in archival FFPE tissues, regardless of the age of the block, compared with matched frozen specimen, ranging from maximal preservation of fatty acids (78%) to loss of the majority of peptides and steroids. Generally, FFPE samples showed a decrease of metabolites with functional groups, such as carboxamide. As an adjunct technique, metabolic profiles were also obtained in situ from FFPE tissue sections where metabolites were extracted in a manner that preserves tissue architecture. Despite the fact that selected metabolites were not retained after processing, global metabolic profiles obtained from FFPE can be used to predict biologic states and study biologic pathways. These results pave the way for metabolomics-based biomarker discovery/validation utilizing retrospective and clinically annotated FFPE collections.
    Áreas temáticas: Oncology Odontología Molecular biology Medicine (all) Medicina iii Medicina ii Medicina i Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Cell biology Cancer research Biotecnología
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: oscar.yanes@urv.cat
    Identificador del autor: 0000-0003-3695-7157
    Fecha de alta del registro: 2024-09-07
    Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
    Enlace a la fuente original: https://mcr.aacrjournals.org/content/15/4/439
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Molecular Cancer Research. 15 (4): 439-447
    Referencia de l'ítem segons les normes APA: Cacciatore S; Zadra G; Bango C; Penney K; Tyekucheva S; Yanes O; Loda M (2017). Metabolic Profiling in Formalin-Fixed and Paraffin-Embedded Prostate Cancer Tissues. Molecular Cancer Research, 15(4), 439-447. DOI: 10.1158/1541-7786.MCR-16-0262
    DOI del artículo: 10.1158/1541-7786.MCR-16-0262
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2017
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Cancer Research,Cell Biology,Molecular Biology,Oncology
    Oncology
    Odontología
    Molecular biology
    Medicine (all)
    Medicina iii
    Medicina ii
    Medicina i
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Cell biology
    Cancer research
    Biotecnología
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