Autor según el artículo: Cacciatore S; Zadra G; Bango C; Penney K; Tyekucheva S; Yanes O; Loda M

Departamento: Enginyeria Electrònica, Elèctrica i Automàtica

Autor/es de la URV: Yanes Torrado, Óscar

Resumen: Metabolite profiling has significantly contributed to a deeper understanding of the biochemical metabolic networks and pathways in cancer cells. Metabolomics-based biomarker discovery would greatly benefit from the ability to interrogate retrospective annotated clinical specimens archived as formalin-fixed, paraffinembedded (FFPE) material. Mass spectrometry-based metabolomic analysis was performed in matched frozen and FFPE human prostate cancers as well as isogenic prostate cancer cell lines. A total of 352 and 460 metabolites were profiled in human tissues and cell lines, respectively. Classes and physical-chemical characteristics of the metabolites preserved in FFPE material were characterized and related to their preservation or loss following fixation and embedding. Metabolite classes were differentially preserved in archival FFPE tissues, regardless of the age of the block, compared with matched frozen specimen, ranging from maximal preservation of fatty acids (78%) to loss of the majority of peptides and steroids. Generally, FFPE samples showed a decrease of metabolites with functional groups, such as carboxamide. As an adjunct technique, metabolic profiles were also obtained in situ from FFPE tissue sections where metabolites were extracted in a manner that preserves tissue architecture. Despite the fact that selected metabolites were not retained after processing, global metabolic profiles obtained from FFPE can be used to predict biologic states and study biologic pathways. These results pave the way for metabolomics-based biomarker discovery/validation utilizing retrospective and clinically annotated FFPE collections.

Áreas temáticas: Oncology Odontología Molecular biology Medicine (all) Medicina iii Medicina ii Medicina i Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Cell biology Cancer research Biotecnología

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

Direcció de correo del autor: oscar.yanes@urv.cat

Identificador del autor: 0000-0003-3695-7157

Fecha de alta del registro: 2023-07-31

Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion

Enlace a la fuente original: https://mcr.aacrjournals.org/content/15/4/439

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Molecular Cancer Research. 15 (4): 439-447

Referencia de l'ítem segons les normes APA: Cacciatore S; Zadra G; Bango C; Penney K; Tyekucheva S; Yanes O; Loda M (2017). Metabolic Profiling in Formalin-Fixed and Paraffin-Embedded Prostate Cancer Tissues. Molecular Cancer Research, 15(4), 439-447. DOI: 10.1158/1541-7786.MCR-16-0262

DOI del artículo: 10.1158/1541-7786.MCR-16-0262

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2017

Tipo de publicación: Journal Publications