Autor según el artículo: H Julia Ellis, M Carmen Bermudo Redondo, Tanja Suligoj, Beatriz Corte-Real , Paul J Ciclitira
Departamento: Enginyeria Química
Autor/es de la URV: Bermudo Redondo, Maria del Carmen
Palabras clave: Mostres reals Immunoassaig Gluten Gliadin Detecció del gluten
Resumen: Coeliac disease (CD) is an immune mediated condition involving dietary gluten, found in wheat, rye, and barley; avoiding them is the only treatment. We generated a mAb to the immunodominant gliadin epitope alpha-2-gli-57-75 and developed assays to quantify gluten in foods, comparing the results with those from the R5 assay. Competition assays were developed using native gliadin as the competing and coating antigen. Results were compared with those from the Ingenesa Ingezim Gluten ® kit. The mAb recognized prolamins from wheat, spelt, rye and barley. A competition assay that detected gliadin at 0.027ppm in a foodstuff revealed gliadin contamination of several foods labelled gluten-free. Purified wheat starch, used as wheat flour substitute to generate gluten-free foods, contains quantifiable amounts of the immunodominant epitope. The assay is sufficiently sensitive to assess the suitability of foods for coeliac patients and could be used as an alternative to the R5.
Áreas temáticas: Saúde coletiva Nutrição Educação física
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: carmen.bermudo@urv.cat carmen.bermudo@urv.cat carmen.bermudo@urv.cat
Identificador del autor: 0000-0002-4128-9803 0000-0002-4128-9803 0000-0002-4128-9803
Fecha de alta del registro: 2024-01-13
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: http://verizonaonlinepublishing.com/CurrentIssueFoodThree.aspx
Referencia al articulo segun fuente origial: Food And Nutrition Report. 1 (3): 1-7
Referencia de l'ítem segons les normes APA: H Julia Ellis, M Carmen Bermudo Redondo, Tanja Suligoj, Beatriz Corte-Real , Paul J Ciclitira (2016). Gluten Quantification of foods via the Immunodominang Gliadin Epitope. Food And Nutrition Report, 1(3), 1-7. DOI: 10.24218/fnr.2015.11
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI del artículo: 10.24218/fnr.2015.11
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2016
Tipo de publicación: Journal Publications