Autor según el artículo: Orlowska A, Perera PT, Al Kobaisi MA, Dias A, Nguyen HKD, Ghanaati S, Baulin V, Crawford RJ, Ivanova EP

Departamento: Enginyeria Química

Autor/es de la URV: Baulin, Vladimir

Palabras clave: Substratum Proliferation Poly-l-lysine Pc12 neuronal differentiation Neuronal differentiation Nerve growth factor (ngf) Laminin Fibronectin Adhesion pc12 neuronal differentiation nerve growth factor (ngf) laminin fibronectin

Resumen: Cellular attachment plays a vital role in the differentiation of pheochromocytoma (PC12) cells. PC12 cells are noradrenergic clonal cells isolated from the adrenal medulla of Rattus norvegicus and studied extensively as they have the ability to differentiate into sympathetic neuron-like cells. The effect of several experimental parameters including (i) the concentration of nerve growth factor (NGF); (ii) substratum coatings, such as poly-L-lysine (PLL), fibronectin (Fn), and laminin (Lam); and (iii) double coatings composed of PLL/Lam and PLL/Fn on the differentiation process of PC12 cells were studied. Cell morphology was visualised using brightfield phase contrast microscopy, cellular metabolism and proliferation were quantified using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, and the neurite outgrowth and axonal generation of the PC12 cells were evaluated using wide field fluorescence microscopy. It was found that double coatings of PLL/Lam and PLL/Fn supported robust adhesion and a two-fold enhanced neurite outgrowth of PC12 cells when treated with 100 ng/mL of NGF while exhibiting stable metabolic activity, leading to the accelerated generation of axons.

Áreas temáticas: Química Odontología Medicina veterinaria Medicina i Materials science, multidisciplinary Materiais Interdisciplinar General materials science Engenharias iv Engenharias iii Engenharias ii Engenharias i Condensed matter physics Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Astronomia / física Arquitetura, urbanismo e design

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 19961944

Direcció de correo del autor: vladimir.baulin@urv.cat

Identificador del autor: 0000-0003-2086-4271

Fecha de alta del registro: 2023-07-31

Volumen de revista: 11

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://www.mdpi.com/1996-1944/11/1/60

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Materials. 11 (1): 60-

Referencia de l'ítem segons les normes APA: Orlowska A, Perera PT, Al Kobaisi MA, Dias A, Nguyen HKD, Ghanaati S, Baulin V, Crawford RJ, Ivanova EP (2018). The Effect of Coatings and Nerve Growth Factor on Attachment and Differentiation of Pheochromocytoma Cells. Materials, 11(1), 60-. DOI: 10.3390/ma11010060

DOI del artículo: 10.3390/ma11010060

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2018

Página inicial: Article number 60

Tipo de publicación: Journal Publications