Articles producció científica> Enginyeria Química

Dynamic studies of the interaction of a pH responsive, amphiphilic polymer with a DOPC lipid membrane

  • Datos identificativos

    Identificador: imarina:5131908
    Handle: http://hdl.handle.net/20.500.11797/imarina5131908
  • Autores:

    Ramadurai S, Werner M, Slater NKH, Martin A, Baulin VA, Keyes TE.
  • Otros:

    Autor según el artículo: Ramadurai S, Werner M, Slater NKH, Martin A, Baulin VA, Keyes TE.
    Departamento: Enginyeria Química
    e-ISSN: 1744-6848
    Autor/es de la URV: Baulin, Vladimir
    Palabras clave: Poly(l-lysine iso-phthalamide) Peptides Model membranes Lifetime correlation spectroscopy Lateral diffusion Hydrophobic mismatch Fluorescence correlation spectroscopy Cell-membrane Blood-cells Bilayer-membranes
    Resumen: Deeper understanding of the molecular interactions between polymeric materials and the lipid membrane is important across a range of applications from permeation for drug delivery to encapsulation for immuno-evasion. Using highly fluidic microcavity supported lipid bilayers, we studied the interactions between amphiphilic polymer PP50 and a DOPC lipid bilayer. As the PP50 polymer is pH responsive the studies were carried out at pH 6.5, 7.05 and 7.5, corresponding to fully, partly protonated (pH = pKa = 7.05) and fully ionized states of the polymer, respectively. Fluorescence correlation spectroscopy (FCS) using both labelled lipid and polymer revealed the PP50 associates with the bilayer interface across all pHs where its diffusion along the interface is impeded. Both FCS and electrochemical impedance spectroscopy (EIS) data indicate that the PP50 does not penetrate fully into the bilayer core but rather forms a layer at the bilayer aqueous interface reflected in increased resistance and decreased capacitance of the bilayer on PP50 binding. The extent of these effects and the dynamics of binding are influenced by pH, increasing with decreasing pH. These experimental trends concurred with coarse grained Monte Carlo simulations of polymer-bilayer interactions wherein a model hydrophilic polymer backbone grafted with side chains of varying hydrophobicity, to mimic the effect of varying pH, was simulated based on the bond fluctuation model with explicit solvent. Simulation results showed that with increasing hydrophobicity, the polymer penetrated deeper into the contacting bilayer leaflet of the membrane suppressing, consistent with EIS data, solvent permeation and that a full insertion of the polymer into the bilayer core is not necessary for suppression of permeability.
    Áreas temáticas: Química Polymer science Physics, multidisciplinary Materials science, multidisciplinary Materiais Interdisciplinar General chemistry Farmacia Engenharias iv Engenharias iii Engenharias ii Condensed matter physics Ciências biológicas ii Ciências biológicas i Ciências agrárias i Chemistry, physical Chemistry (miscellaneous) Chemistry (all) Biotecnología Astronomia / física
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1744-683X
    Direcció de correo del autor: vladimir.baulin@urv.cat
    Identificador del autor: 0000-0003-2086-4271
    Fecha de alta del registro: 2023-02-18
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://pubs.rsc.org/en/content/articlelanding/2017/sm/c6sm02645a#!divAbstract
    URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Soft Matter. 13 (20): 3690-3700
    Referencia de l'ítem segons les normes APA: Ramadurai S, Werner M, Slater NKH, Martin A, Baulin VA, Keyes TE. (2017). Dynamic studies of the interaction of a pH responsive, amphiphilic polymer with a DOPC lipid membrane. Soft Matter, 13(20), 3690-3700. DOI: 10.1039/C6SM02645A
    DOI del artículo: 10.1039/c6sm02645a
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2017
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Chemistry (Miscellaneous),Chemistry, Physical,Condensed Matter Physics,Materials Science, Multidisciplinary,Physics, Multidisciplinary,Polymer Science
    Poly(l-lysine iso-phthalamide)
    Peptides
    Model membranes
    Lifetime correlation spectroscopy
    Lateral diffusion
    Hydrophobic mismatch
    Fluorescence correlation spectroscopy
    Cell-membrane
    Blood-cells
    Bilayer-membranes
    Química
    Polymer science
    Physics, multidisciplinary
    Materials science, multidisciplinary
    Materiais
    Interdisciplinar
    General chemistry
    Farmacia
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Condensed matter physics
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Chemistry, physical
    Chemistry (miscellaneous)
    Chemistry (all)
    Biotecnología
    Astronomia / física
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