Autor según el artículo: Margalef R, Sisquella M, Bosque M, Romeu C, Mayoral O, Monterde S, Priego M, Guerra-Perez R, Ortiz N, Tomàs J, Santafe M
Departamento: Ciències Mèdiques Bàsiques Medicina i Cirurgia
Autor/es de la URV: Monterde Pérez, Sonia / Ortiz Castellón, Nicolau Antoni / Santafé Martínez, Manuel
Palabras clave: Spontaneous acetylcholine release Myofascial trigger point Myofascial pain syndrome Endplate noise Animal model myofascial trigger point myofascial pain syndrome endplate noise animal model
Resumen: Myofascial pain syndrome is one of the most common forms of muscle pain. In this syndrome, pain is originated by the so-called trigger points, which consists of a set of palpable contraction knots in the muscle. It has been proposed that a high, spontaneous neurotransmission may be involved in the generation of these contraction knots. To confirm this hypothesis, we exposed mouse muscles to an anticholinesterasic agent to increase the neurotransmision in the synaptic cleft in two different conditions, in vivo and ex vivo experiments. Using intracellular recordings, a sharp increase in the spontaneous neurotransmission in the levator auris longus muscle and a lower increase in the diaphragm muscle could be seen. Likewise, electromyography recordings reveal an elevated endplate noise in gastrocnemius muscle of treated animals. These changes are associated with structural changes such as abundant neuromuscular contracted zones observed by rhodaminated α-bungarotoxin and the presence of abundant glycosaminoglycans around the contraction knots, as shown by Alcian PAS staining. In a second set of experiments, we aimed at demonstrating that the increases in the neurotransmission reproduced most of the clinical signs associated to a trigger point. We exposed rats to the anticholinesterase agent neostigmine, and 30 min afterward we observed the presence of palpable taut bands, the echocardiographic presence of contraction knots, and local twitch responses upon needle stimulation. In summary, we demonstrated that increased neurotransmission induced trigger points in both rats and mice, as evidenced by glycosaminoglycans around the contraction zones as a novel hallmark of this pathology. NEW & NOTEWORTHY In rodents, when neostigmine was injected subcutaneously, the neuromuscular neurotransmission increased, and several changes can be observed: an elevated endplate noise compared with normal endplate noise, as evidenced by electromyographyc recording; many muscular fibers with contraction knots (narrower sarcomeres and locally thickened muscle fiber) surrounded by infiltration of connective tissue like glycosaminoglycans molecules; and palpable taut bands and local twitch responses upon needle stimulation. Several of these signs are also observed in humans with muscle pain.
Áreas temáticas: Zootecnia / recursos pesqueiros Sports science Sport sciences Saúde coletiva Psicología Physiology (medical) Physiology Nutrição Medicine (miscellaneous) Medicine (all) Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Geociências General medicine Farmacia Engenharias iv Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Biotecnología Biodiversidade Astronomia / física
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 87507587
Direcció de correo del autor: nicolauantoni.ortiz@urv.cat manuel.santafe@urv.cat sonia.monterde@urv.cat
Identificador del autor: 0000-0002-5462-5108 0000-0001-5580-3757
Fecha de alta del registro: 2023-07-31
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://journals.physiology.org/doi/pdfplus/10.1152/japplphysiol.00248.2018
Referencia al articulo segun fuente origial: Journal Of Applied Physiology. 126 (1): 160-169
Referencia de l'ítem segons les normes APA: Margalef R, Sisquella M, Bosque M, Romeu C, Mayoral O, Monterde S, Priego M, Guerra-Perez R, Ortiz N, Tomàs J, Santafe M (2019). Experimental myofascial trigger point creation in rodents. Journal Of Applied Physiology, 126(1), 160-169. DOI: 10.1152/japplphysiol.00248.2018
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI del artículo: 10.1152/japplphysiol.00248.2018
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2019
Tipo de publicación: Journal Publications
Repositori URV