Autor según el artículo: Gonzalez-Quilen, Carlos; Gil-Cardoso, Katherine; Gines, Iris; Beltran-Debon, Raul; Pinent, Montserrat; Ardevol, Anna; Terra, Ximena; Blay, M Teresa
Departamento: Bioquímica i Biotecnologia
e-ISSN: 2072-6643
Autor/es de la URV: Ardévol Grau, Anna / Beltrán Debón, Raúl Alejandro / Blay Olivé, Maria Teresa / GINÉS MIR, IRIS / GONZALEZ QUILEN, CARLOS ALBERTO / Pinent Armengol, Montserrat / Terra Barbadora, Ximena
Palabras clave: Vitis Ussing chamber Transepithelial electrical resistance Tight junction Supplementation Seeds Rich Rats, wistar Rats Proanthocyanidins Obesity Liver Intestinal diseases Insulin-resistance Inflammation High-fat diet Gut microbiota Gut Flavan-3-ol Female Extract Endotoxemia Dose-response relationship, drug Diet Barrier function Animals ussing chamber tight junction obesity inflammation gut flavan-3-ol
Resumen: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. We evaluated the effectiveness of pharmacological doses of grape-seed proanthocyanidin extract (GSPE) in reversing intestinal barrier alterations and local inflammation in female Wistar rats fed a long-term obesogenic diet. Animals were fed a 17-week cafeteria diet (CAF diet), supplemented with daily GSPE doses (100 or 500 mg kg-1 body weight) during the final two weeks. CAF diet enhanced the intestinal permeation of an orally administered marker (ovalbumin, OVA) and increased the plasma levels of tumor necrosis factor-α (TNF-α) and lipopolysaccharides (LPS) in 2-3-fold. Ex vivo Ussing chamber assays showed a 55-70% reduction in transepithelial electrical resistance (TEER) and increased the TNF-α secretions in both small and large intestinal sections with a 25-fold increment in the ileum. Ileal tissues also presented a 4-fold increase of myeloperoxidase (MPO) activity. Both GSPE-treatments were able to restitute TEER values in the ileum and colon and to reduce plasma LPS to basal levels without a dose-dependent effect. However, effects on the OVA permeation and TNF-α secretion were dose and section-specific. GSPE also reduced ileal MPO activity and upregulated claudin 1 gene expression. This study provides evidence of the efficacy of GSPE-supplementation ameliorating diet-induced intestinal dysfunction and metabolic endotoxemia when administered at the end of a long-term obesogenic diet.
Áreas temáticas: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Planejamento urbano e regional / demografia Nutrition and dietetics Nutrition & dietetics Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Food science Farmacia Engenharias iv Engenharias ii Enfermagem Educação física Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 20726643
Direcció de correo del autor: anna.ardevol@urv.cat ximena.terra@urv.cat mteresa.blay@urv.cat montserrat.pinent@urv.cat raul.beltran@urv.cat
Identificador del autor: 0000-0003-0156-7538 0000-0003-1043-5844 0000-0002-6256-9847 0000-0003-3550-5378 0000-0001-9691-1906
Fecha de alta del registro: 2024-10-19
Volumen de revista: 11
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://www.mdpi.com/2072-6643/11/5/979
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Nutrients. 11 (5): 979-
Referencia de l'ítem segons les normes APA: Gonzalez-Quilen, Carlos; Gil-Cardoso, Katherine; Gines, Iris; Beltran-Debon, Raul; Pinent, Montserrat; Ardevol, Anna; Terra, Ximena; Blay, M Teresa (2019). Grape-seed proanthocyanidins are able to reverse intestinal dysfunction and metabolic endotoxemia induced by a cafeteria diet in wistar rats. Nutrients, 11(5), 979-. DOI: 10.3390/nu11050979
DOI del artículo: 10.3390/nu11050979
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2019
Tipo de publicación: Journal Publications