Articles producció científica> Medicina i Cirurgia

Fecal Gluten Peptides Reveal Limitations of Serological Tests and Food Questionnaires for Monitoring Gluten-Free Diet in Celiac Disease Patients

  • Datos identificativos

    Identificador: imarina:5908388
    Autores:
    Comino IFernández-Bañares FEsteve MOrtigosa LCastillejo GFambuena BRibes-Koninckx CSierra CRodríguez-Herrera ASalazar JCaunedo ÁMarugán-Miguelsanz JGarrote JVivas SLo Iacono ONuñez AVaquero LVegas ACrespo LFernández-Salazar LArranz EJiménez-García VAntonio Montes-Cano MEspín BGalera AValverde JGirón FBolonio MMillán ACerezo FGuajardo CAlberto JRosinach MSegura VLeón FMarinich JMuñoz-Suano ARomero-Gómez MCebolla ÁSousa C
    Resumen:
    Objectives: Treatment for celiac disease (CD) is a lifelong strict gluten-free diet (GFD). Patients should be followed-up with dietary interviews and serology as CD markers to ensure adherence to the diet. However, none of these methods offer an accurate measure of dietary compliance. Our aim was to evaluate the measurement of gluten immunogenic peptides (GIP) in stools as a marker of GFD adherence in CD patients and compare it with traditional methods of GFD monitoring. Methods: We performed a prospective, nonrandomized, multicenter study including 188 CD patients on GFD and 84 healthy controls. Subjects were given a dietary questionnaire and fecal GIP quantified by enzyme-linked immunosorbent assay (ELISA). Serological anti-tissue transglutaminase (anti-tTG) IgA and anti-deamidated gliadin peptide (anti-DGP) IgA antibodies were measured simultaneously.Results: Of the 188 celiac patients, 56 (29.8%) had detectable GIP levels in stools. There was significant association between age and GIP in stools that revealed increasing dietary transgressions with advancing age (39.2% in subjects ≥13 years old) and with gender in certain age groups (60% in men ≥13 years old). No association was found between fecal GIP and dietary questionnaire or anti-tTG antibodies. However, association was detected between GIP and anti-DGP antibodies, although 46 of the 53 GIP stool-positive patients were negative for anti-DGP.Conclusions: Detection of gluten peptides in stools reveals limitations of traditional methods for monitoring GFD in celiac patients. The GIP ELISA enables direct and quantitative assessment of gluten exposure early after ingestion and could aid in the diagnosis and clinical management of nonresponsive CD and refractory CD. Trial registration number NCT02711397.
  • Otros:

    Autor según el artículo: Comino I; Fernández-Bañares F; Esteve M; Ortigosa L; Castillejo G; Fambuena B; Ribes-Koninckx C; Sierra C; Rodríguez-Herrera A; Salazar J; Caunedo Á; Marugán-Miguelsanz J; Garrote J; Vivas S; Lo Iacono O; Nuñez A; Vaquero L; Vegas A; Crespo L; Fernández-Salazar L; Arranz E; Jiménez-García V; Antonio Montes-Cano M; Espín B; Galera A; Valverde J; Girón F; Bolonio M; Millán A; Cerezo F; Guajardo C; Alberto J; Rosinach M; Segura V; León F; Marinich J; Muñoz-Suano A; Romero-Gómez M; Cebolla Á; Sousa C
    Departamento: Medicina i Cirurgia
    Autor/es de la URV: Castillejo De Villasante, Gemma
    Palabras clave: @infoAeu @residentesaeu @uroweb Etiqueta «#» Hashtag
    Resumen: Objectives: Treatment for celiac disease (CD) is a lifelong strict gluten-free diet (GFD). Patients should be followed-up with dietary interviews and serology as CD markers to ensure adherence to the diet. However, none of these methods offer an accurate measure of dietary compliance. Our aim was to evaluate the measurement of gluten immunogenic peptides (GIP) in stools as a marker of GFD adherence in CD patients and compare it with traditional methods of GFD monitoring. Methods: We performed a prospective, nonrandomized, multicenter study including 188 CD patients on GFD and 84 healthy controls. Subjects were given a dietary questionnaire and fecal GIP quantified by enzyme-linked immunosorbent assay (ELISA). Serological anti-tissue transglutaminase (anti-tTG) IgA and anti-deamidated gliadin peptide (anti-DGP) IgA antibodies were measured simultaneously.Results: Of the 188 celiac patients, 56 (29.8%) had detectable GIP levels in stools. There was significant association between age and GIP in stools that revealed increasing dietary transgressions with advancing age (39.2% in subjects ≥13 years old) and with gender in certain age groups (60% in men ≥13 years old). No association was found between fecal GIP and dietary questionnaire or anti-tTG antibodies. However, association was detected between GIP and anti-DGP antibodies, although 46 of the 53 GIP stool-positive patients were negative for anti-DGP.Conclusions: Detection of gluten peptides in stools reveals limitations of traditional methods for monitoring GFD in celiac patients. The GIP ELISA enables direct and quantitative assessment of gluten exposure early after ingestion and could aid in the diagnosis and clinical management of nonresponsive CD and refractory CD. Trial registration number NCT02711397.
    Áreas temáticas: Medicine (all) Medicina iii Medicina ii Medicina i Hepatology General medicine Gastroenterology & hepatology Gastroenterology Ciências biológicas i Biotecnología
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 00029270
    Direcció de correo del autor: gemma.castillejo@urv.cat
    Fecha de alta del registro: 2024-09-07
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://pubmed.ncbi.nlm.nih.gov/27644734/
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: American Journal Of Gastroenterology. 111 (10): 1456-1465
    Referencia de l'ítem segons les normes APA: Comino I; Fernández-Bañares F; Esteve M; Ortigosa L; Castillejo G; Fambuena B; Ribes-Koninckx C; Sierra C; Rodríguez-Herrera A; Salazar J; Caunedo Á; (2016). Fecal Gluten Peptides Reveal Limitations of Serological Tests and Food Questionnaires for Monitoring Gluten-Free Diet in Celiac Disease Patients. American Journal Of Gastroenterology, 111(10), 1456-1465. DOI: 10.1038/ajg.2016.439
    DOI del artículo: 10.1038/ajg.2016.439
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2016
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Gastroenterology,Gastroenterology & Hepatology,Hepatology
    Medicine (all)
    Medicina iii
    Medicina ii
    Medicina i
    Hepatology
    General medicine
    Gastroenterology & hepatology
    Gastroenterology
    Ciências biológicas i
    Biotecnología
  • Documentos:

  • Cerca a google

    Search to google scholar