Autor según el artículo: Magrina, Ivan; Jauset-Rubio, Miriam; Ortiz, Mayreli; Tomaso, Herbert; Simonova, Anna; Hocek, Michal; O'Sullivan, Ciara K
Departamento: Enginyeria Química
Autor/es de la URV: Jauset Rubio, Miriam / Magriñá Lobato, Ivan / O'SULLIVAN, CIARA KATHLEEN / Ortíz Rodríguez, Mayreli
Palabras clave: Triphosphates Redox label Real Number Nucleotides Identification Gene Francisella-tularensis Assays Amplification
Resumen: We report the duplex amplification of two plasmid DNA markers involved in the virulence of Bacillus anthracis, CAP and PAG, and the direct electrochemical detection of these amplicons. The method consists of the simultaneous amplification of the two targets in a single-pot reaction via polymerase chain reaction (PCR) using tailed primers and ferrocene-labeled dATP. Following amplification, the PCR products hybridize to probes immobilized on electrodes in a microfabricated electrode array chip. The incorporated ferrocene labeled dATP is then detected using square wave voltammetry. We evaluated the effect of electrolyte cations, anions, and concentration to condense, bend, and shrink double-stranded DNA and their effect on the intensity of the ferrocene signal. We obtained detection limits 40.8 and 3.4 fM for CAP and PAG targets, respectively. We successfully developed a method to detect the presence of both targets in genomic DNA extracted from real samples.
Áreas temáticas: Química Interdisciplinar General chemistry General chemical engineering Engenharias ii Ciências agrárias i Chemistry, multidisciplinary Chemistry (miscellaneous) Chemistry (all) Chemical engineering (miscellaneous) Chemical engineering (all)
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 24701343
Direcció de correo del autor: mayreli.ortiz@urv.cat miriam.jauset@urv.cat mayreli.ortiz@urv.cat
Identificador del autor: 0000-0002-9423-0055 0000-0002-9943-6132 0000-0002-9423-0055
Fecha de alta del registro: 2024-10-26
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://pubs.acs.org/doi/abs/10.1021/acsomega.9b02890
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Acs Omega. 4 (26): 21900-21908
Referencia de l'ítem segons les normes APA: Magrina, Ivan; Jauset-Rubio, Miriam; Ortiz, Mayreli; Tomaso, Herbert; Simonova, Anna; Hocek, Michal; O'Sullivan, Ciara K (2019). Duplex Electrochemical DNA Sensor to Detect Bacillus anthracis CAP and PAG DNA Targets Based on the Incorporation of Tailed Primers and Ferrocene-Labeled dATP. Acs Omega, 4(26), 21900-21908. DOI: 10.1021/acsomega.9b02890
DOI del artículo: 10.1021/acsomega.9b02890
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2019
Tipo de publicación: Journal Publications
Repositori URV