Articles producció científica> Enginyeria Química

Addressing the Analytical Challenges for the Detection of Ciguatoxins Using an Electrochemical Biosensor

  • Datos identificativos

    Identificador: imarina:6249287
    Autores:
    Leonardo, SandraGaiani, GretaTsumuraya, TakeshiHirama, MasahiroTurquet, JeanSagrista, NuriaRambla-Alegre, MariaFlores, CintiaCaixach, JosepDiogene, JorgeO'Sullivan, Ciara KAlcaraz, CarlesCampas, Monica
    Resumen:
    The importance of ciguatoxins (CTXs) in seafood safety and their emerging occurrence in locations far away from tropical areas highlight the need for simple and low-cost methods for the sensitive and rapid detection of these potent marine toxins to protect seafood consumers. Herein, an electrochemical immunosensor for the detection of CTXs is presented. A sandwich configuration is proposed, using magnetic beads (MBs) as immobilization supports for two capture antibodies, with their combination facilitating the detection of CTX1B, CTX3C, 54-deoxyCTX1B, and 51-hydroxyCTX3C. PoIyHRP-streptavidin is used for the detection of the biotinylated detector antibody. Experimental conditions are first optimized using colorimetry, and these conditions are subsequently used for electrochemical detection on electrode arrays. Limits of detection at the pg/mL level are achieved for CTX1B and 5 1-hydroxyCTX3C. The applicability of the immunosensor to the analysis of fish samples is demonstrated, attaining detection of CTX1B at contents as low as 0.01 mu g/kg and providing results in correlation with those obtained using mouse bioassay (MBA) and cell-based assay (CBA), and confirmed by liquid chromatography coupled to high-resolution mass spectrometry (LC-ESI-HRMS). This user-friendly bioanalytical tool for the rapid detection of CTXs can be used to mitigate ciguatera risk and contribute to the protection of consumer health.
  • Otros:

    Autor según el artículo: Leonardo, Sandra; Gaiani, Greta; Tsumuraya, Takeshi; Hirama, Masahiro; Turquet, Jean; Sagrista, Nuria; Rambla-Alegre, Maria; Flores, Cintia; Caixach, Josep; Diogene, Jorge; O'Sullivan, Ciara K; Alcaraz, Carles; Campas, Monica
    Departamento: Enginyeria Química
    Autor/es de la URV: CAMPAS HOMS, MONICA / O'SULLIVAN, CIARA KATHLEEN
    Palabras clave: Islands ne atlantic toxins sandwich immunoassay ocean monoclonal-antibodies immunoassay stick test gambierdiscus fish ciguatera 1st report
    Resumen: The importance of ciguatoxins (CTXs) in seafood safety and their emerging occurrence in locations far away from tropical areas highlight the need for simple and low-cost methods for the sensitive and rapid detection of these potent marine toxins to protect seafood consumers. Herein, an electrochemical immunosensor for the detection of CTXs is presented. A sandwich configuration is proposed, using magnetic beads (MBs) as immobilization supports for two capture antibodies, with their combination facilitating the detection of CTX1B, CTX3C, 54-deoxyCTX1B, and 51-hydroxyCTX3C. PoIyHRP-streptavidin is used for the detection of the biotinylated detector antibody. Experimental conditions are first optimized using colorimetry, and these conditions are subsequently used for electrochemical detection on electrode arrays. Limits of detection at the pg/mL level are achieved for CTX1B and 5 1-hydroxyCTX3C. The applicability of the immunosensor to the analysis of fish samples is demonstrated, attaining detection of CTX1B at contents as low as 0.01 mu g/kg and providing results in correlation with those obtained using mouse bioassay (MBA) and cell-based assay (CBA), and confirmed by liquid chromatography coupled to high-resolution mass spectrometry (LC-ESI-HRMS). This user-friendly bioanalytical tool for the rapid detection of CTXs can be used to mitigate ciguatera risk and contribute to the protection of consumer health.
    Áreas temáticas: Química Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Enfermagem Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, analytical Biotecnología Biodiversidade Astronomia / física Analytical chemistry
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 0003-2700
    Direcció de correo del autor: monica.campas@urv.cat
    Identificador del autor: 0000-0002-1220-7100
    Página final: 4865
    Fecha de alta del registro: 2024-10-26
    Volumen de revista: 92
    Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
    Enlace a la fuente original: https://pubs.acs.org/doi/abs/10.1021/acs.analchem.9b04499
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Analytical Chemistry. 92 (7): 4858-4865
    Referencia de l'ítem segons les normes APA: Leonardo, Sandra; Gaiani, Greta; Tsumuraya, Takeshi; Hirama, Masahiro; Turquet, Jean; Sagrista, Nuria; Rambla-Alegre, Maria; Flores, Cintia; Caixach, (2020). Addressing the Analytical Challenges for the Detection of Ciguatoxins Using an Electrochemical Biosensor. Analytical Chemistry, 92(7), 4858-4865. DOI: 10.1021/acs.analchem.9b04499
    DOI del artículo: 10.1021/acs.analchem.9b04499
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2020
    Página inicial: 4858
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Analytical Chemistry,Chemistry, Analytical
    Islands ne atlantic
    toxins
    sandwich immunoassay
    ocean
    monoclonal-antibodies
    immunoassay stick test
    gambierdiscus
    fish
    ciguatera
    1st report
    Química
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Geociências
    General medicine
    Farmacia
    Ensino
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Enfermagem
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, analytical
    Biotecnología
    Biodiversidade
    Astronomia / física
    Analytical chemistry
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