Articles producció científica> Ciències Mèdiques Bàsiques

Specific circulating microRNA signature of bicuspid aortic valve disease

  • Datos identificativos

    Identificador: imarina:6389099
    Autores:
    Martínez-Micaelo, NBeltrán-Debón, RBaiges, IFaiges, MAlegret, JM
    Resumen:
    © 2017 The Author(s). Background: We aimed to determine the circulating miRNA expression profile associated with BAV and aortic dilation to provide diagnostic and prognostic biomarkers for BAV and/or aortic dilation. Methods and results: We applied a miRNome-wide microarray approach using plasma samples (n = 24) from healthy tricuspid aortic valve individuals, BAV patients and BAV patients with aortic dilation to compare and identify the specific miRNAs associated with BAV and aortic dilation. In a second stage, the expression patterns of the miRNA candidates were validated by RT-qPCR in an independent cohort (n = 43). The miRNA microarray data and RT-qPCR analyses revealed that the expression levels of circulating miR-122, miR-130a and miR-486 are significantly influenced by the morphology of the aortic valve (bicuspid/tricuspid) and could be functionally involved in the regulation of TGF-β1 signalling. Furthermore, the expression pattern of miR-718 in the plasma was strongly influenced by dilation of the ascending aorta. miR-718 expression was inversely correlated with the aortic diameter (R = -0.63, p = 3.1 × 10-5) and was an independent predictor of aortic dilation (β = -0.41, p = 0.022). The genes targeted by miR-718 are involved in the regulation of vascular remodelling. Conclusions: We propose that miR-122, miR-130a, miR-486 and miR-718 are new molecular features associated with BAV and aortic dilation principally by the activation of TGF-β1 pathway and vascular remodelling mediated by VEGF signalling pathways.
  • Otros:

    Autor según el artículo: Martínez-Micaelo, N; Beltrán-Debón, R; Baiges, I; Faiges, M; Alegret, JM
    Departamento: Ciències Mèdiques Bàsiques Bioquímica i Biotecnologia
    Autor/es de la URV: Alegret Colomé, Josep Maria / BAIGES BLANCO, ISABEL MARIA / Beltrán Debón, Raúl Alejandro / Faiges Borràs, Marta Jesús / Martínez Micaelo, Nieves Beatriz
    Palabras clave: Smooth-muscle-cells Mir-718 Micrornas Microrna Identification Discovery Dilation Differential expression Calcification Bicuspid aortic valve Beta Bav bicuspid aortic valve Aortopathy Aortic dilation Angiogenesis
    Resumen: © 2017 The Author(s). Background: We aimed to determine the circulating miRNA expression profile associated with BAV and aortic dilation to provide diagnostic and prognostic biomarkers for BAV and/or aortic dilation. Methods and results: We applied a miRNome-wide microarray approach using plasma samples (n = 24) from healthy tricuspid aortic valve individuals, BAV patients and BAV patients with aortic dilation to compare and identify the specific miRNAs associated with BAV and aortic dilation. In a second stage, the expression patterns of the miRNA candidates were validated by RT-qPCR in an independent cohort (n = 43). The miRNA microarray data and RT-qPCR analyses revealed that the expression levels of circulating miR-122, miR-130a and miR-486 are significantly influenced by the morphology of the aortic valve (bicuspid/tricuspid) and could be functionally involved in the regulation of TGF-β1 signalling. Furthermore, the expression pattern of miR-718 in the plasma was strongly influenced by dilation of the ascending aorta. miR-718 expression was inversely correlated with the aortic diameter (R = -0.63, p = 3.1 × 10-5) and was an independent predictor of aortic dilation (β = -0.41, p = 0.022). The genes targeted by miR-718 are involved in the regulation of vascular remodelling. Conclusions: We propose that miR-122, miR-130a, miR-486 and miR-718 are new molecular features associated with BAV and aortic dilation principally by the activation of TGF-β1 pathway and vascular remodelling mediated by VEGF signalling pathways.
    Áreas temáticas: Odontología Nutrição Medicine, research & experimental Medicine (miscellaneous) Medicine (all) Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar General medicine General biochemistry,genetics and molecular biology Farmacia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Biotecnología Biochemistry, genetics and molecular biology (miscellaneous) Biochemistry, genetics and molecular biology (all)
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 14795876
    Direcció de correo del autor: neus.martinez@urv.cat marta.faiges@estudiants.urv.cat josepmaria.alegret@urv.cat raul.beltran@urv.cat
    Identificador del autor: 0000-0002-6117-5512 0000-0001-9691-1906
    Fecha de alta del registro: 2024-08-03
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-017-1176-x
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Journal Of Translational Medicine. 15 (1): 76-
    Referencia de l'ítem segons les normes APA: Martínez-Micaelo, N; Beltrán-Debón, R; Baiges, I; Faiges, M; Alegret, JM (2017). Specific circulating microRNA signature of bicuspid aortic valve disease. Journal Of Translational Medicine, 15(1), 76-. DOI: 10.1186/s12967-017-1176-x
    DOI del artículo: 10.1186/s12967-017-1176-x
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2017
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Biochemistry, Genetics and Molecular Biology (Miscellaneous),Medicine (Miscellaneous),Medicine, Research & Experimental
    Smooth-muscle-cells
    Mir-718
    Micrornas
    Microrna
    Identification
    Discovery
    Dilation
    Differential expression
    Calcification
    Bicuspid aortic valve
    Beta
    Bav bicuspid aortic valve
    Aortopathy
    Aortic dilation
    Angiogenesis
    Odontología
    Nutrição
    Medicine, research & experimental
    Medicine (miscellaneous)
    Medicine (all)
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Interdisciplinar
    General medicine
    General biochemistry,genetics and molecular biology
    Farmacia
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Biotecnología
    Biochemistry, genetics and molecular biology (miscellaneous)
    Biochemistry, genetics and molecular biology (all)
  • Documentos:

  • Cerca a google

    Search to google scholar