Articles producció científica> Ciències Mèdiques Bàsiques

PCR Based Detection of Phase Variable Genes in Pakistani Based Clinical Helicobacter pylori Strains

  • Datos identificativos

    Identificador: imarina:6478320
    Handle: https://hdl.handle.net/20.500.11797/imarina6478320
    Autores:
    Ahmad, HajraKhan, ImadKhan, Fida MuhammadRahman, Amjad UrMughal, ImranMurad, WaheedKhan, SalmanRahman, Faiz UrAhmad, FaisalAhmad, Sajjad
    Resumen:
    Background: The distribution pattern of phase-variable genes varies from strain to strain and from region to region. The present study was carried out to investigate the distribution pattern of phase-variable genes within Pakistan-based Helicobacter pylori strains and to analyze and compare them with strains prevalent in other parts of the world. Objectives: To determine the distribution pattern of phase-variable genes in H. pylori strains circulating in Pakistan. Patients and Methods: Biopsy samples were collected from 85 symptomatic patients suffering from various upper gastrointestinal tract symptoms. The biopsy specimens were chopped, then inoculated on H. pylori-specific media and incubated in a Campylobacter Gas Generating kit. Positive isolates were further confirmed via staining and biochemical procedures. Primers were designed for five phase-variable genes using OligoCalc, an oligonucleotide properties calculator (version 3.26) according to parameters stipulated in the literature. Polymerase chain reaction (PCR) was performed on all positive isolates to determine the presence or absence of phase-variable genes. Results: On culturing, the prevalence of H. pylori infections in the samples was 44.7%. The prevalence was higher in females than in males, and it increased with age. PCR amplification revealed that the hsdR gene was present in 79% of samples, while the mod and beta-subunit genes were present in 16% and 30% of samples, respectively. The streptococcal M protein gene was found in 79%, while the fliP gene was prevalent in 56%. Conclusions: The distribution patterns of phase-variable genes in Pakistani H. pylori strains were found to be somewhat different. The dominant prevalence of the hsdR gene was an interesting finding, considering its role in bacterial

  • Otros:

    Autor según el artículo: Ahmad, Hajra Khan, Imad Khan, Fida Muhammad Rahman, Amjad Ur Mughal, Imran Murad, Waheed Khan, Salman Rahman, Faiz Ur Ahmad, Faisal Ahmad, Sajjad
    Departamento: Ciències Mèdiques Bàsiques
    Autor/es de la URV: UR, FAIZ
    Palabras clave: Sequence Restriction modification system Phase-variable genes Pcr Infection Helicobacter pylori Genome Diversity Adaptation phase-variable genes pcr helicobacter pylori
    Resumen: Background: The distribution pattern of phase-variable genes varies from strain to strain and from region to region. The present study was carried out to investigate the distribution pattern of phase-variable genes within Pakistan-based Helicobacter pylori strains and to analyze and compare them with strains prevalent in other parts of the world. Objectives: To determine the distribution pattern of phase-variable genes in H. pylori strains circulating in Pakistan. Patients and Methods: Biopsy samples were collected from 85 symptomatic patients suffering from various upper gastrointestinal tract symptoms. The biopsy specimens were chopped, then inoculated on H. pylori-specific media and incubated in a Campylobacter Gas Generating kit. Positive isolates were further confirmed via staining and biochemical procedures. Primers were designed for five phase-variable genes using OligoCalc, an oligonucleotide properties calculator (version 3.26) according to parameters stipulated in the literature. Polymerase chain reaction (PCR) was performed on all positive isolates to determine the presence or absence of phase-variable genes. Results: On culturing, the prevalence of H. pylori infections in the samples was 44.7%. The prevalence was higher in females than in males, and it increased with age. PCR amplification revealed that the hsdR gene was present in 79% of samples, while the mod and beta-subunit genes were present in 16% and 30% of samples, respectively. The streptococcal M protein gene was found in 79%, while the fliP gene was prevalent in 56%. Conclusions: The distribution patterns of phase-variable genes in Pakistani H. pylori strains were found to be somewhat different. The dominant prevalence of the hsdR gene was an interesting finding, considering its role in bacterial defense in both micro- and macroenvironments.
    Áreas temáticas: Microbiology (medical) Microbiology Infectious diseases
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 20083645
    Direcció de correo del autor: faiz.urrahman@irta.cat
    Identificador del autor: 0000-0001-6965-2355
    Fecha de alta del registro: 2021-04-17
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Referencia al articulo segun fuente origial: Jundishapur Journal Of Microbiology. 9 (7): e31824-
    Referencia de l'ítem segons les normes APA: Ahmad, Sajjad; Ahmad, Faisal; Rahman, Faiz Ur; Khan, Salman; Murad, Waheed; Mughal, Imran; Rahman, Amjad Ur; Khan, Fida Muhammad; Khan, Imad; Ahmad, H (2016). PCR Based Detection of Phase Variable Genes in Pakistani Based Clinical Helicobacter pylori Strains. Jundishapur Journal Of Microbiology, 9(7), e31824-. DOI: 10.5812/jjm.31824
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2016
    Tipo de publicación: Journal Publications

  • Palabras clave:

    Infectious Diseases,Microbiology,Microbiology (Medical)
    Sequence
    Restriction modification system
    Phase-variable genes
    Pcr
    Infection
    Helicobacter pylori
    Genome
    Diversity
    Adaptation
    phase-variable genes
    pcr
    helicobacter pylori
    Microbiology (medical)
    Microbiology
    Infectious diseases

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