Articles producció científica> Medicina i Cirurgia

Obesity determines the immunophenotypic profile and functional characteristics of human mesenchymal stem cells from adipose tissue

  • Datos identificativos

    Identificador: imarina:6656291
    Autores:
    Pachón-Peña GSerena CEjarque MPetriz JDuran XOliva-Olivera WSimó RTinahones FJFernández-Veledo SVendrell J
    Resumen:
    © AlphaMed Press 2016. Adipose tissue is a major source ofmesenchymal stemcells (MSCs), which possess a variety of properties that make them ideal candidates for regenerative and immunomodulatory therapies. Here, we compared the immunophenotypic profile of human adipose-derived stem cells (hASCs) from lean and obese individuals, and explored its relationship with the apparent altered plasticity of hASCs.Wealso hypothesized that persistent hypoxia treatment of cultured hASCsmay be necessary but not sufficient to drive significant changes inmature adipocytes. hASCswere obtained fromsubcutaneous adipose tissue of healthy, adult, female donors undergoing abdominal plastic surgery: lean (n = 8; bodymass index [BMI]: 23 ± kg/m2) and obese (n = 8; BMI: 35 ± kg/m2). Cell surface marker expression, proliferation and migration capacity, and adipogenic differentiation potential of cultured hASCs at two different oxygen conditions were studied. Compared with lean-derived hASCs, obese-derived hASCs demonstrated increased proliferation and migration capacity but decreased lipid droplet accumulation, correlating with a higher expression of human leukocyte antigen (HLA)-II and cluster of differentiation (CD) 106 and lower expression of CD29. Of interest, adipogenic differentiation modified CD106, CD49b, HLA-ABC surface protein expression, which was dependent on the donor’s BMI. Additionally, low oxygen tension increased proliferation and migration of lean but not obese hASCs, which correlated with an altered CD36 and CD49b immunophenotypic profile. In summary, the differences observed in proliferation, migration, and differentiation capacity in obese hASCs occurred in parallel with changes in cell surfacemarkers, both under basal conditions and during differentiation. Therefore, obesit
  • Otros:

    Autor según el artículo: Pachón-Peña G; Serena C; Ejarque M; Petriz J; Duran X; Oliva-Olivera W; Simó R; Tinahones FJ; Fernández-Veledo S; Vendrell J
    Departamento: Medicina i Cirurgia Ciències Mèdiques Bàsiques
    Autor/es de la URV: Fernandez Veledo, Sonia / Serena Perelló, Carolina / VENDRELL FERRE, JOAN / Vendrell Ortega, Juan José
    Palabras clave: Stromal cells Plasticity Oxygen-tension Osteogenic differentiation Obesity Morbid-obesity International-society Insulin-resistance In-vitro Immunophenotype Human mesenchymal stem cells Gene-expression Endothelial progenitor cells Bone-marrow Adipogenic differentiation
    Resumen: © AlphaMed Press 2016. Adipose tissue is a major source ofmesenchymal stemcells (MSCs), which possess a variety of properties that make them ideal candidates for regenerative and immunomodulatory therapies. Here, we compared the immunophenotypic profile of human adipose-derived stem cells (hASCs) from lean and obese individuals, and explored its relationship with the apparent altered plasticity of hASCs.Wealso hypothesized that persistent hypoxia treatment of cultured hASCsmay be necessary but not sufficient to drive significant changes inmature adipocytes. hASCswere obtained fromsubcutaneous adipose tissue of healthy, adult, female donors undergoing abdominal plastic surgery: lean (n = 8; bodymass index [BMI]: 23 ± kg/m2) and obese (n = 8; BMI: 35 ± kg/m2). Cell surface marker expression, proliferation and migration capacity, and adipogenic differentiation potential of cultured hASCs at two different oxygen conditions were studied. Compared with lean-derived hASCs, obese-derived hASCs demonstrated increased proliferation and migration capacity but decreased lipid droplet accumulation, correlating with a higher expression of human leukocyte antigen (HLA)-II and cluster of differentiation (CD) 106 and lower expression of CD29. Of interest, adipogenic differentiation modified CD106, CD49b, HLA-ABC surface protein expression, which was dependent on the donor’s BMI. Additionally, low oxygen tension increased proliferation and migration of lean but not obese hASCs, which correlated with an altered CD36 and CD49b immunophenotypic profile. In summary, the differences observed in proliferation, migration, and differentiation capacity in obese hASCs occurred in parallel with changes in cell surfacemarkers, both under basal conditions and during differentiation. Therefore, obesity is an important determinant of stem cell function independent of oxygen tension.
    Áreas temáticas: Medicine (miscellaneous) Medicine (all) Medicina ii Medicina i General medicine Developmental biology Ciências biológicas iii Ciências biológicas ii Cell biology Cell & tissue engineering
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 21576564
    Direcció de correo del autor: carolina.serena@urv.cat sonia.fernandez@urv.cat juanjose.vendrell@urv.cat
    Identificador del autor: 0000-0003-2906-3788 0000-0002-6994-6115
    Fecha de alta del registro: 2024-11-02
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://stemcellsjournals.onlinelibrary.wiley.com/doi/full/10.5966/sctm.2015-0161
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Stem Cells Translational Medicine. 5 (4): 464-475
    Referencia de l'ítem segons les normes APA: Pachón-Peña G; Serena C; Ejarque M; Petriz J; Duran X; Oliva-Olivera W; Simó R; Tinahones FJ; Fernández-Veledo S; Vendrell J (2016). Obesity determines the immunophenotypic profile and functional characteristics of human mesenchymal stem cells from adipose tissue. Stem Cells Translational Medicine, 5(4), 464-475. DOI: 10.5966/sctm.2015-0161
    DOI del artículo: 10.5966/sctm.2015-0161
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2016
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Cell & Tissue Engineering,Cell Biology,Developmental Biology,Medicine (Miscellaneous)
    Stromal cells
    Plasticity
    Oxygen-tension
    Osteogenic differentiation
    Obesity
    Morbid-obesity
    International-society
    Insulin-resistance
    In-vitro
    Immunophenotype
    Human mesenchymal stem cells
    Gene-expression
    Endothelial progenitor cells
    Bone-marrow
    Adipogenic differentiation
    Medicine (miscellaneous)
    Medicine (all)
    Medicina ii
    Medicina i
    General medicine
    Developmental biology
    Ciências biológicas iii
    Ciências biológicas ii
    Cell biology
    Cell & tissue engineering
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