Autor según el artículo: Casanova-Marti, Angela; Gonzalez-Abuin, Noemi; Serrano, Joan; Blay, Maria Teresa; Terra, Ximena; Frost, Gary; Pinent, Montserrat; Ardevol, Anna
Departamento: Bioquímica i Biotecnologia
Autor/es de la URV: Ardévol Grau, Anna / Blay Olivé, Maria Teresa / CASANOVA MARTÍ, ANGELA / Pinent Armengol, Montserrat / Terra Barbadora, Ximena
Palabras clave: Secretion Receptors, g-protein-coupled Proanthocyanidins Proanthocyanidins
Peptide yy Organoids Mucin-2 Muc2 protein, mouse Mice, inbred c57bl Lgr5 protein, mouse Inhibition Incretin Ileum Htas2r14 Gut Green tea Grape seed proanthocyanidins Grape seed extract Glucagon-like peptide-1 Glucagon-like peptide 1 Gastrointestinal hormones Gallic acid Enteroendocrine Enterocytes Endocrine cell Decrease Cell differentiation Catechin Bitter-taste receptor Animals Activation incretin glucagon-like peptide-1 enteroendocrine
Resumen: Scope A grape-seed proanthocyanidin extract (GSPE) interacts at the intestinal level, enhancing glucagon-like peptide-1 (GLP-1) and peptide YY (PYY) release, which modulate appetite and glucose homeostasis. Thus, enhancing L-cell numbers could be a strategy to promote hormone production, providing a potential strategy for obesity and type-2 diabetes mellitus (T2DM) treatment. Methods and results Mice ileum organoids are used to evaluate the long-term effects of GSPE and two of its main components, epicatechin (EC) and gallic acid (GA), on intestinal differentiation. Hormone levels are determined using RIA and ELISA kits, and gene expression of transcription factors involved in intestinal cell differentiation, as well as markers of different cell types, are assessed by real-time qPCR. GSPE upregulates enterohormone gene expression and content, as well as the pan-endocrine marker chromogranin A. GSPE also modulates the temporal gene expression profile of early and late transcription factors involved in L-cell differentiation. Furthermore, GSPE upregulates goblet cell (Muc2) and enterocyte (sucraseisomaltase) markers, while downregulating stem cell markers (Lgr5+). Although EC and GA modified enterohormone release, they do not reproduce GSPE effects on transcription factor's profile. Conclusions This study shows the potential role of GSPE in promoting enteroendocrine differentiation, effect that is not mediated by EC or GA.
Áreas temáticas: Saúde coletiva Química Nutrição Medicina veterinaria Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Educação física Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología Biotechnology Astronomia / física
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 1613-4125
Direcció de correo del autor: anna.ardevol@urv.cat ximena.terra@urv.cat mteresa.blay@urv.cat montserrat.pinent@urv.cat
Identificador del autor: 0000-0003-0156-7538 0000-0003-1043-5844 0000-0002-6256-9847 0000-0003-3550-5378
Fecha de alta del registro: 2024-10-19
Versión del articulo depositado: info:eu-repo/semantics/submittedVersion
Enlace a la fuente original: https://onlinelibrary.wiley.com/doi/abs/10.1002/mnfr.202000303
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Molecular Nutrition & Food Research. 64 (2000303): e2000303-
Referencia de l'ítem segons les normes APA: Casanova-Marti, Angela; Gonzalez-Abuin, Noemi; Serrano, Joan; Blay, Maria Teresa; Terra, Ximena; Frost, Gary; Pinent, Montserrat; Ardevol, Anna (2020). Long Term Exposure to a Grape Seed Proanthocyanidin Extract Enhances L-Cell Differentiation in Intestinal Organoids. Molecular Nutrition & Food Research, 64(2000303), e2000303-. DOI: 10.1002/mnfr.202000303
DOI del artículo: 10.1002/mnfr.202000303
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2020
Tipo de publicación: Journal Publications