Articles producció científica> Ciències Mèdiques Bàsiques

Performance of the digene LQ, RH and PS HPVs genotyping systems on clinical samples and comparison with HC2 and PCR-based Linear Array

  • Datos identificativos

    Identificador: imarina:835446
    Autores:
    Godinez, Jose MTous, SaraBaixeras, NuriaMoreno-Crespi, JudithAlejo, MariaLejeune, MaryleneBravo, Ignacio GBosch, F Xavierde Sanjose, Silvia
    Resumen:
    Certain Human Papillomaviruses (HPVs) are the infectious agents involved in cervical cancer development. Detection of HPVs DNA is part of the cervical cancer screening protocols and HPVs genotyping has been proposed for its inclusion in these preventive programs. The aim of this study was to evaluate three novel genotyping tests, namely Qiagen LQ, RH and PS, in clinical samples with and without abnormalities. For this, 305 cervical samples were processed and the results of the evaluated techniques were compared with those obtained in the HPVs diagnostic process in our lab, by using HC2 and Linear Array (LA) technologies.The concordances and kappa statistics (k) for each technique compared with HC2 were 98.69% (k = 0.94) for LQ, 98.03% (k = 0.91) for RH and 91.80% (k = 0.82) for PS. There was a very good agreement in HPVs type-specific concordance for the most prevalent types HPV16 (kappa range = 0.83-0.90), HPV18 (k.r.= 0.74-0.80) and HPV45 (k.r.= 0.82-0.90).The three tests showed an overall good concordance for HPVs detection when compared with HR-HC2 system. LQ and RH rendered lower detection rate for multiple infections than LA genotyping. However, our understanding of the clinical significance of multiple HPVs infections is still incomplete and therefore the relevance of the lower ability to detect multiple infections needs to be evaluated.
  • Otros:

    Autor según el artículo: Godinez, Jose M; Tous, Sara; Baixeras, Nuria; Moreno-Crespi, Judith; Alejo, Maria; Lejeune, Marylene; Bravo, Ignacio G; Bosch, F Xavier; de Sanjose, Silvia
    Departamento: Ciències Mèdiques Bàsiques
    Autor/es de la URV: Lejeune, Marylène Marie
    Palabras clave: Papillomaviruses Hpv Genotyping Cervical cancer screening
    Resumen: Certain Human Papillomaviruses (HPVs) are the infectious agents involved in cervical cancer development. Detection of HPVs DNA is part of the cervical cancer screening protocols and HPVs genotyping has been proposed for its inclusion in these preventive programs. The aim of this study was to evaluate three novel genotyping tests, namely Qiagen LQ, RH and PS, in clinical samples with and without abnormalities. For this, 305 cervical samples were processed and the results of the evaluated techniques were compared with those obtained in the HPVs diagnostic process in our lab, by using HC2 and Linear Array (LA) technologies.The concordances and kappa statistics (k) for each technique compared with HC2 were 98.69% (k = 0.94) for LQ, 98.03% (k = 0.91) for RH and 91.80% (k = 0.82) for PS. There was a very good agreement in HPVs type-specific concordance for the most prevalent types HPV16 (kappa range = 0.83-0.90), HPV18 (k.r.= 0.74-0.80) and HPV45 (k.r.= 0.82-0.90).The three tests showed an overall good concordance for HPVs detection when compared with HR-HC2 system. LQ and RH rendered lower detection rate for multiple infections than LA genotyping. However, our understanding of the clinical significance of multiple HPVs infections is still incomplete and therefore the relevance of the lower ability to detect multiple infections needs to be evaluated.
    Áreas temáticas: Saúde coletiva Química Oncology Medicina iii Medicina ii Medicina i Interdisciplinar Infectious diseases Immunology Farmacia Epidemiology Enfermagem Ciências biológicas iii Ciências biológicas i Ciências ambientais Cancer research Biotecnología Biodiversidade
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 17509378
    Direcció de correo del autor: marylenemarie.lejeune@urv.cat
    Identificador del autor: 0000-0001-8441-9404
    Fecha de alta del registro: 2024-10-12
    Volumen de revista: 6
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://infectagentscancer.biomedcentral.com/articles/10.1186/1750-9378-6-23
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Infectious Agents And Cancer. 6 (1): 23-
    Referencia de l'ítem segons les normes APA: Godinez, Jose M; Tous, Sara; Baixeras, Nuria; Moreno-Crespi, Judith; Alejo, Maria; Lejeune, Marylene; Bravo, Ignacio G; Bosch, F Xavier; de Sanjose, S (2011). Performance of the digene LQ, RH and PS HPVs genotyping systems on clinical samples and comparison with HC2 and PCR-based Linear Array. Infectious Agents And Cancer, 6(1), 23-. DOI: 10.1186/1750-9378-6-23
    DOI del artículo: 10.1186/1750-9378-6-23
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2011
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Cancer Research,Epidemiology,Immunology,Infectious Diseases,Oncology
    Papillomaviruses
    Hpv
    Genotyping
    Cervical cancer screening
    Saúde coletiva
    Química
    Oncology
    Medicina iii
    Medicina ii
    Medicina i
    Interdisciplinar
    Infectious diseases
    Immunology
    Farmacia
    Epidemiology
    Enfermagem
    Ciências biológicas iii
    Ciências biológicas i
    Ciências ambientais
    Cancer research
    Biotecnología
    Biodiversidade
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