Autor según el artículo: Rodríguez-Calvo R; Samino S; Girona J; Martínez-Micaelo N; Ràfols P; García-Altares M; Guaita-Esteruelas S; Junza A; Heras M; Yanes O; Correig X; Masana L

Departamento: Enginyeria Electrònica, Elèctrica i Automàtica Medicina i Cirurgia

Autor/es de la URV: Correig Blanchar, Francesc Xavier / Garcia-Altares Pérez, Maria / Girona Tell, Josefa / GUAITA ESTERUELAS, SANDRA / HERAS IBAÑEZ, MERCEDES / Junza Martínez, Alexandra / Martínez Micaelo, Nieves Beatriz / Masana Marín, Luis / Ràfols Soler, Pere / SAMINO GENÉ, SARA / Yanes Torrado, Óscar

Palabras clave: Stress Steatosis Steatohepatitis Pathogenesis Nafld Liver triglycerides Lipidome Insulin-resistance Inhibition Inflammation Fabp4 Endoplasmic-reticulum De-novo lipogenesis Alpha Adipokines Acid-binding protein nafld lipidome inflammation fabp4 adipokines

Resumen: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. An imbalance between hepatic fatty acid uptake and removal results in ectopic fat accumulation, which leads to non-alcoholic fatty liver disease (NAFLD). The amount and type of accumulated triglycerides seem to play roles in NAFLD progression; however, a complete understanding of how triglycerides contribute to NAFLD evolution is lacking. Our aim was to evaluate triglyceride accumulation in NAFLD in a murine model and its associations with molecular mechanisms involved in liver damage and adipose tissue-liver cross talk by employing lipidomic and molecular imaging techniques. C57BL/6J mice fed a high-fat diet (HFD) for 12 weeks were used as a NAFLD model. Standard-diet (STD)-fed animals were used as controls. Standard liver pathology was assessed using conventional techniques. The liver lipidome was analyzed by liquid chromatography–mass spectrometry (LC–MS) and laser desorption/ionization–mass spectrometry (LDI–MS) tissue imaging. Liver triglycerides were identified by MS/MS. The transcriptome of genes involved in intracellular lipid metabolism and inflammation was assessed by RT-PCR. Plasma leptin, resistin, adiponectin, and FABP4 levels were determined using commercial kits. HFD-fed mice displayed increased liver lipid content. LC–MS analyses identified 14 triglyceride types that were upregulated in livers from HFD-fed animals. Among these 14 types, 10 were identified in liver cross sections by LDI–MS tissue imaging. The accumulation of these triglycerides was associated with the upregulation of lipogenesis and inflammatory genes and the downregulation of β-oxidation genes. Interestingly, the levels of plasma FABP4, but not of other adipokines, were positively associated with 8 of these triglycerides in HFD-fed mice but not in STD-fed mice. Our findings suggest a putative role of FABP4 in the liver-adipose tissue cross talk in NAFLD.

Áreas temáticas: Química Molecular biology Materiais General medicine Farmacia Ensino Biochemistry & molecular biology Biochemistry

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 2218273X

Direcció de correo del autor: xavier.correig@urv.cat maria.garcia-altares@urv.cat alexandra.junza@urv.cat josefa.girona@urv.cat oscar.yanes@urv.cat neus.martinez@urv.cat pere.rafols@urv.cat luis.masana@urv.cat josefa.girona@urv.cat

Identificador del autor: 0000-0002-6902-3054 0000-0001-7205-0419 0000-0002-6267-8779 0000-0003-3695-7157 0000-0002-9240-4058 0000-0002-0789-4954 0000-0002-6267-8779

Fecha de alta del registro: 2023-02-27

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://www.mdpi.com/2218-273X/10/9/1275

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Biomolecules. 10 (9): 1-15

Referencia de l'ítem segons les normes APA: Rodríguez-Calvo R; Samino S; Girona J; Martínez-Micaelo N; Ràfols P; García-Altares M; Guaita-Esteruelas S; Junza A; Heras M; Yanes O; Correig X; Masa (2020). Hepatic lipidomics and molecular imaging in a murine non-alcoholic fatty liver disease model: Insights into molecular mechanisms. Biomolecules, 10(9), 1-15. DOI: 10.3390/biom10091275

DOI del artículo: 10.3390/biom10091275

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2020

Tipo de publicación: Journal Publications