Autor según el artículo: Capellades, Jordi; Junza, Alexandra; Samino, Sara; Brunner, Julia S; Schabbauer, Gernot; Vinaixa, Maria; Yanes, Oscar
Departamento: Enginyeria Electrònica, Elèctrica i Automàtica
e-ISSN: 1520-6882
Autor/es de la URV: De la Varga Pastor, Aitana / Junza Martínez, Alexandra / SAMINO GENÉ, SARA / Vinaixa Crevillent, Maria / Yanes Torrado, Óscar
Resumen: © Isotopic-labeling experiments have been valuable to monitor the flux of metabolic reactions in biological systems, which is crucial to understand homeostatic alterations with disease. Experimental determination of metabolic fluxes can be inferred from a characteristic rearrangement of stable isotope tracers (e.g., 13C or 15N) that can be detected by mass spectrometry (MS). Metabolites measured are generally members of well-known metabolic pathways, and most of them can be detected using both gas chromatography (GC)-MS and liquid chromatography (LC)-MS. In here, we show that GC methods coupled to chemical ionization (CI) MS have a clear advantage over alternative methodologies due to GC's superior chromatography separation efficiency and the fact that CI is a soft ionization technique that yields identifiable protonated molecular ion peaks. We tested diverse GC-CI-MS setups, including methane and isobutane reagent gases, triple quadrupole (QqQ) MS in SIM mode, or selected ion clusters using optimized narrow windows (∼10 Da) in scan mode, and standard full scan methods using high resolution GC-(q)TOF and GC-Orbitrap systems. Isobutane as a reagent gas in combination with both low-resolution (LR) and high-resolution (HR) MS showed the best performance, enabling precise detection of isotopologues in most metabolic intermediates of central carbon metabolism. Finally, with the aim of overcoming manual operations, we developed an R-based tool called isoSCAN that automatically quantifies all isotopologues of intermediate metabolites of glycolysis, TCA cycle, amino acids, pentose phosphate pathway, and urea cycle, from LRMS and HRMS data.
Áreas temáticas: Química Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Enfermagem Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, analytical Biotecnología Biodiversidade Astronomia / física Analytical chemistry
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 0003-2700
Direcció de correo del autor: maria.vinaixa@urv.cat oscar.yanes@urv.cat alexandra.junza@urv.cat maria.vinaixa@urv.cat aitana.delavarga@urv.cat
Identificador del autor: 0000-0001-9804-0171 0000-0003-3695-7157 0000-0001-7205-0419 0000-0001-9804-0171 0000-0002-3124-4409
Fecha de alta del registro: 2024-10-12
Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
Enlace a la fuente original: https://pubs.acs.org/doi/10.1021/acs.analchem.0c02998
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Analytical Chemistry. 93 (3): 1242-1248
Referencia de l'ítem segons les normes APA: Capellades, Jordi; Junza, Alexandra; Samino, Sara; Brunner, Julia S; Schabbauer, Gernot; Vinaixa, Maria; Yanes, Oscar (2021). Exploring the Use of Gas Chromatography Coupled to Chemical Ionization Mass Spectrometry (GC-CI-MS) for Stable Isotope Labeling in Metabolomics. Analytical Chemistry, 93(3), 1242-1248. DOI: 10.1021/acs.analchem.0c02998
DOI del artículo: 10.1021/acs.analchem.0c02998
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2021
Tipo de publicación: Journal Publications