Autor según el artículo: Salazar, Jose Gregorio; Marsillach, Judit; Reverte, Ingrid; Mackness, Bharti; Mackness, Michael; Joven, Jorge; Camps, Jordi; Colomina, Maria Teresa;

Departamento: Psicologia

Autor/es de la URV: Colomina Fosch, Maria Teresa / Joven Maried, Jorge / REVERTÉ SOLER, INGRID

Palabras clave: Tg2576 mice S disease Paraoxonases Oxidative stress Neurons Microglia Hippocampus Brain Astrocytes Amyloid-β Amyloid-? Alzheimer’ Alzheimer’s disease

Resumen: Background: Brain oxidative lipid damage and inflammation are common in neurodegenerative diseases such as Alzheimer's disease (AD). Paraoxonase-1 and -3 (PON1 and PON3) protein expression was demonstrated in tissue with no PON1 or PON3 gene expression. In the present study, we examine differences in PON1 and PON3 protein expression in the brain of a mouse model of AD. Methods: we used peroxidase- and fluorescence-based immunohistochemistry in five brain regions (olfactory bulb, forebrain, posterior midbrain, hindbrain and cerebellum) of transgenic (Tg2576) mice with the Swedish mutation (KM670/671NL) responsible for a familial form of AD and corresponding wild-type mice. Results: We found intense PON1 and PON3-positive staining in star-shaped cells surrounding A beta plaques in all the studied Tg2576 mouse-brain regions. Although we could not colocalize PON1 and PON3 with astrocytes (star-shaped cells in the brain), we found some PON3 colocalization with microglia. Conclusions: These results suggest that (1) PON1 and PON3 cross the blood-brain barrier in discoidal high-density lipoproteins (HDLs) and are transferred to specific brain-cell types; and (2) PON1 and PON3 play an important role in preventing oxidative stress and lipid peroxidation in particular brain-cell types (likely to be glial cells) in AD pathology and potentially in other neurodegenerative diseases as well.

Áreas temáticas: Química Physiology Molecular biology Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Engenharias ii Clinical biochemistry Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry, medicinal Cell biology Biotecnología Biodiversidade Biochemistry & molecular biology Biochemistry

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

Direcció de correo del autor: mariateresa.colomina@urv.cat jorge.joven@urv.cat

Identificador del autor: 0000-0002-5619-4874 0000-0003-2749-4541

Fecha de alta del registro: 2023-08-05

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://www.mdpi.com/2076-3921/10/3/339

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Antioxidants. 10 (3): 1-12

Referencia de l'ítem segons les normes APA: Salazar, Jose Gregorio; Marsillach, Judit; Reverte, Ingrid; Mackness, Bharti; Mackness, Michael; Joven, Jorge; Camps, Jordi; Colomina, Maria Teresa; (2021). Paraoxonase-1 and-3 Protein Expression in the Brain of the Tg2576 Mouse Model of Alzheimer's Disease. Antioxidants, 10(3), 1-12. DOI: 10.3390/antiox10030339

DOI del artículo: 10.3390/antiox10030339

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2021

Tipo de publicación: Journal Publications