Autor según el artículo: Salazar, Jose Gregorio; Marsillach, Judit; Reverte, Ingrid; Mackness, Bharti; Mackness, Michael; Joven, Jorge; Camps, Jordi; Colomina, Maria Teresa;
Departamento: Psicologia
Autor/es de la URV: Colomina Fosch, Maria Teresa / Joven Maried, Jorge / MARSILLACH LÓPEZ, JUDIT / REVERTÉ SOLER, INGRID
Palabras clave: Tg2576 mice S disease Paraoxonases Oxidative stress Neurons Microglia Hippocampus Brain Astrocytes Amyloid-β Amyloid-? Alzheimer’ Alzheimer’s disease
Resumen: Background: Brain oxidative lipid damage and inflammation are common in neurodegenerative diseases such as Alzheimer's disease (AD). Paraoxonase-1 and -3 (PON1 and PON3) protein expression was demonstrated in tissue with no PON1 or PON3 gene expression. In the present study, we examine differences in PON1 and PON3 protein expression in the brain of a mouse model of AD. Methods: we used peroxidase- and fluorescence-based immunohistochemistry in five brain regions (olfactory bulb, forebrain, posterior midbrain, hindbrain and cerebellum) of transgenic (Tg2576) mice with the Swedish mutation (KM670/671NL) responsible for a familial form of AD and corresponding wild-type mice. Results: We found intense PON1 and PON3-positive staining in star-shaped cells surrounding A beta plaques in all the studied Tg2576 mouse-brain regions. Although we could not colocalize PON1 and PON3 with astrocytes (star-shaped cells in the brain), we found some PON3 colocalization with microglia. Conclusions: These results suggest that (1) PON1 and PON3 cross the blood-brain barrier in discoidal high-density lipoproteins (HDLs) and are transferred to specific brain-cell types; and (2) PON1 and PON3 play an important role in preventing oxidative stress and lipid peroxidation in particular brain-cell types (likely to be glial cells) in AD pathology and potentially in other neurodegenerative diseases as well.
Áreas temáticas: Química Physiology Molecular biology Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Engenharias ii Clinical biochemistry Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry, medicinal Cell biology Biotecnología Biodiversidade Biochemistry & molecular biology Biochemistry
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: jorge.joven@urv.cat mariateresa.colomina@urv.cat
Identificador del autor: 0000-0003-2749-4541 0000-0002-5619-4874
Fecha de alta del registro: 2024-10-12
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://www.mdpi.com/2076-3921/10/3/339
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Antioxidants. 10 (3): 339-12
Referencia de l'ítem segons les normes APA: Salazar, Jose Gregorio; Marsillach, Judit; Reverte, Ingrid; Mackness, Bharti; Mackness, Michael; Joven, Jorge; Camps, Jordi; Colomina, Maria Teresa; (2021). Paraoxonase-1 and-3 Protein Expression in the Brain of the Tg2576 Mouse Model of Alzheimer's Disease. Antioxidants, 10(3), 339-12. DOI: 10.3390/antiox10030339
DOI del artículo: 10.3390/antiox10030339
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2021
Tipo de publicación: Journal Publications