Articles producció científica> Enginyeria Química

Improvement of Biohydrogen and usable chemical products from glycerol by co-culture of Enterobacter sph1 and Citrobacter Freundii h3 using different supports as surface immobilization

  • Datos identificativos

    Identificador: imarina:9228414
    Handle: http://hdl.handle.net/20.500.11797/imarina9228414
  • Autores:

    Maru BT
    Lopez F
    Medina F
    Constantí M
  • Otros:

    Autor según el artículo: Maru BT; Lopez F; Medina F; Constantí M
    Departamento: Enginyeria Química
    Autor/es de la URV: Constantí Garriga, Magdalena / López Bonillo, Francisco / Medina Cabello, Francisco
    Palabras clave: Support Silica gel Maghemite Glycerol Fermentative hydrogen-production Enterobacter Citrobacter freundii Biohydrogen Alumina Activated carbon support silica gel metabolism maghemite glucose ferrous iron enterobacter energy balances dark fermentation citrobacter freundii biohydrogen biofilms bacteria alumina aerogenes activated-carbon activated carbon
    Resumen: Glycerol is a by-product of biodiesel production in a yield of about 10% (w/w). The present study aims to improve the dark fermentation of glycerol by surface immobilization of microorganisms on supports. Four different supports were used—maghemite (Fe2O3), activated carbon (AC), silica gel (SiO2), and alumina (-Al2O3)—on which a newly isolated co-culture of Enterobacter spH1 and Citrobacter freundii, H3, was immobilized. The effect of iron species on dark fermentation was also studied by impregnation on AC and SiO2. The fermentative metabolites were mainly ethanol, 1,3-propanediol, lactate, H2 and CO2. The production rate (Rmax,i) and product yield (Yi) were elucidated by modeling using the Gompertz equation for the batch dark fermentation kinetics (maximum product formation (Pmax,i): (i) For each of the supports, H2 production (mmol/L) and yield (mol H2/mol glycerol consumed) increased in the following order: FC < -Al2O3 < Fe2O3 < SiO2 < Fe/SiO2 < AC < Fe/AC. (ii) Ethanol production (mmol/L) increased in the following order: FC < Fe2O3 < -Al2O3 < SiO2 < Fe/SiO2 < Fe/AC < AC, and yield (mol EtOH/mol glycerol consumed) increased in the following order: FC < Fe2O3 < Fe/AC < Fe/SiO2 < SiO2 < AC < -Al2O3. (iii) 1,3-propanediol production (mmol/L) and yield (mol 1,3PDO/mol glycerol consumed) increased in the following order: -Al2O3 < SiO2 < Fe/SiO2 < AC < Fe2O3 < Fe/AC < FC. (iv) Lactate production(mmol/L) and yield (mol Lactate/mol glycerol consumed) increased in the following order: -Al2O3 < SiO2 < AC < Fe/SiO2 < Fe/AC < Fe2O3 < FC. The study shows that in all cases, glycerol conversion was higher when the support assisted culture was used. It is noted that glycerol conversion and H2 production were dependent on the specific surface area of the support. H2 production clearly increased with the Fe2O3, Al2O3, SiO2 and AC supports. H2 production on the iron-impregnated AC and SiO2 supports was higher than on the corresponding bare supports. These results indicate that the support enhances the productivity of H2, perhaps because of specific surface area attachment, biofilm formation of the microorganisms and activation of the hydrogenase enzyme by iron species.
    Áreas temáticas: Plant science Food science Biotechnology & applied microbiology Biochemistry, genetics and molecular biology (miscellaneous)
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: francesc.medina@urv.cat francisco.lopez@urv.cat magdalena.constanti@urv.cat
    Identificador del autor: 0000-0002-3111-1542 0000-0001-5280-8018 0000-0002-3547-4199
    Fecha de alta del registro: 2023-02-23
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.mdpi.com/2311-5637/7/3/154
    URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Fermentation. 7 (3):
    Referencia de l'ítem segons les normes APA: Maru BT; Lopez F; Medina F; Constantí M (2021). Improvement of Biohydrogen and usable chemical products from glycerol by co-culture of Enterobacter sph1 and Citrobacter Freundii h3 using different supports as surface immobilization. Fermentation, 7(3), -. DOI: 10.3390/FERMENTATION7030154
    DOI del artículo: 10.3390/FERMENTATION7030154
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2021
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Biochemistry, Genetics and Molecular Biology (Miscellaneous),Biotechnology & Applied Microbiology,Food Science,Plant Science
    Support
    Silica gel
    Maghemite
    Glycerol
    Fermentative hydrogen-production
    Enterobacter
    Citrobacter freundii
    Biohydrogen
    Alumina
    Activated carbon
    support
    silica gel
    metabolism
    maghemite
    glucose
    ferrous iron
    enterobacter
    energy balances
    dark fermentation
    citrobacter freundii
    biohydrogen
    biofilms
    bacteria
    alumina
    aerogenes
    activated-carbon
    activated carbon
    Plant science
    Food science
    Biotechnology & applied microbiology
    Biochemistry, genetics and molecular biology (miscellaneous)
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