Articles producció científica> Enginyeria Química

Aptamer Sandwich Assay for the Detection of SARS-CoV-2 Spike Protein Antigen

  • Datos identificativos

    Identificador: imarina:9241959
    Handle: http://hdl.handle.net/20.500.11797/imarina9241959
  • Autores:

    Svobodova, Marketa
    Skouridou, Vasso
    Jauset-Rubio, Miriam
    Vieitez, Irene
    Fernandez-Villar, Alberto
    Cabrera Alvargonzalez, Jorge Julio
    Poveda, Eva
    Benavent Bofill, Clara
    Sans, Teresa
    Bashammakh, Abdulaziz
    Alyoubi, Abdulrahman O.
    O'Sullivan, Ciara K.
  • Otros:

    Autor según el artículo: Svobodova, Marketa; Skouridou, Vasso; Jauset-Rubio, Miriam; Vieitez, Irene; Fernandez-Villar, Alberto; Cabrera Alvargonzalez, Jorge Julio; Poveda, Eva; Benavent Bofill, Clara; Sans, Teresa; Bashammakh, Abdulaziz; Alyoubi, Abdulrahman O.; O'Sullivan, Ciara K.;
    Departamento: Enginyeria Química
    Autor/es de la URV: Jauset Rubio, Miriam / O'SULLIVAN, CIARA KATHLEEN / Skouridou, Vasoula / SVOBODOVÁ, MARKÉTA
    Palabras clave: Sensitive detection Selection Receptor-binding domain In-vitro Evolution Enzyme Discovery Coronavirus sensitive detection selection in-vitro evolution enzyme discovery coronavirus
    Resumen: The novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) emerged at the end of 2019, resulting in the ongoing COVID-19 pandemic. The high transmissibility of the virus and the substantial number of asymptomatic individuals have led to an exponential rise in infections worldwide, urgently requiring global containment strategies. Reverse transcription-polymerase chain reaction is the gold standard for the detection of SARS-CoV-2 infections. Antigen tests, targeting the spike (S) or nucleocapsid (N) viral proteins, are considered as complementary tools. Despite their shortcomings in terms of sensitivity and specificity, antigen tests could be deployed for the detection of potentially contagious individuals with high viral loads. In this work, we sought to develop a sandwich aptamer-based assay for the detection of the S protein of SARS-CoV-2. A detailed study on the binding properties of aptamers to the receptor-binding domain of the S protein in search of aptamer pairs forming a sandwich is presented. Screening of aptamer pairs and optimization of assay conditions led to the development of a laboratory-based sandwich assay able to detect 21 ng/mL (270 pM) of the protein with negligible cross-reactivity with the other known human coronaviruses. The detection of 375 pg of the protein in viral transport medium demonstrates the compatibility of the assay with clinical specimens. Finally, successful detection of the S antigen in nasopharyngeal swab samples collected from suspected patients further establishes the suitability of the assay for screening purposes as a complementary tool to assist in the control of the pandemic.
    Áreas temáticas: Química Interdisciplinar General chemistry General chemical engineering Engenharias ii Ciências agrárias i Chemistry, multidisciplinary Chemistry (miscellaneous) Chemistry (all) Chemical engineering (miscellaneous) Chemical engineering (all)
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: miriam.jauset@urv.cat vasoula.skouridou@urv.cat
    Identificador del autor: 0000-0002-9943-6132 0000-0002-9712-5429
    Fecha de alta del registro: 2023-02-19
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://pubs.acs.org/doi/10.1021/acsomega.1c05521
    URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Acs Omega. 6 (51): 35657-35666
    Referencia de l'ítem segons les normes APA: Svobodova, Marketa; Skouridou, Vasso; Jauset-Rubio, Miriam; Vieitez, Irene; Fernandez-Villar, Alberto; Cabrera Alvargonzalez, Jorge Julio; Poveda, Eva (2021). Aptamer Sandwich Assay for the Detection of SARS-CoV-2 Spike Protein Antigen. Acs Omega, 6(51), 35657-35666. DOI: 10.1021/acsomega.1c05521
    DOI del artículo: 10.1021/acsomega.1c05521
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2021
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Chemical Engineering (Miscellaneous),Chemistry (Miscellaneous),Chemistry, Multidisciplinary
    Sensitive detection
    Selection
    Receptor-binding domain
    In-vitro
    Evolution
    Enzyme
    Discovery
    Coronavirus
    sensitive detection
    selection
    in-vitro
    evolution
    enzyme
    discovery
    coronavirus
    Química
    Interdisciplinar
    General chemistry
    General chemical engineering
    Engenharias ii
    Ciências agrárias i
    Chemistry, multidisciplinary
    Chemistry (miscellaneous)
    Chemistry (all)
    Chemical engineering (miscellaneous)
    Chemical engineering (all)
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