Articles producció científica> Enginyeria Química

Gene regulatory network inference in long-lived C. elegans reveals modular properties that are predictive of novel aging genes

  • Datos identificativos

    Identificador: imarina:9243284
    Autores:
    Suriyalaksh, ManusnanRaimondi, CeliaMains, AbrahamSegonds-Pichon, AnneMukhtar, ShahzabeMurdoch, SharleneAldunate, RebecaKrueger, FelixGuimera, RogerAndrews, SimonSales-Pardo, MartaCasanueva, Olivia
    Resumen:
    We design a “wisdom-of-the-crowds” GRN inference pipeline and couple it to complex network analysis to understand the organizational principles governing gene regulation in long-lived glp-1/Notch Caenorhabditis elegans. The GRN has three layers (input, core, and output) and is topologically equivalent to bow-tie/hourglass structures prevalent among metabolic networks. To assess the functional importance of structural layers, we screened 80% of regulators and discovered 50 new aging genes, 86% with human orthologues. Genes essential for longevity—including ones involved in insulin-like signaling (ILS)—are at the core, indicating that GRN's structure is predictive of functionality. We used in vivo reporters and a novel functional network covering 5,497 genetic interactions to make mechanistic predictions. We used genetic epistasis to test some of these predictions, uncovering a novel transcriptional regulator, sup-37, that works alongside DAF-16/FOXO. We present a framework with predictive power that can accelerate discovery in C. elegans and potentially humans.
  • Otros:

    Autor según el artículo: Suriyalaksh, Manusnan; Raimondi, Celia; Mains, Abraham; Segonds-Pichon, Anne; Mukhtar, Shahzabe; Murdoch, Sharlene; Aldunate, Rebeca; Krueger, Felix; Guimera, Roger; Andrews, Simon; Sales-Pardo, Marta; Casanueva, Olivia
    Departamento: Enginyeria Química
    Autor/es de la URV: Guimera Manrique, Roger / Sales Pardo, Marta
    Palabras clave: Life-span Genomics Genetics Bioinformatics metabolism longevity insulin/igf-1 identification expression evolution drosophila daf-16 bow-ties
    Resumen: We design a “wisdom-of-the-crowds” GRN inference pipeline and couple it to complex network analysis to understand the organizational principles governing gene regulation in long-lived glp-1/Notch Caenorhabditis elegans. The GRN has three layers (input, core, and output) and is topologically equivalent to bow-tie/hourglass structures prevalent among metabolic networks. To assess the functional importance of structural layers, we screened 80% of regulators and discovered 50 new aging genes, 86% with human orthologues. Genes essential for longevity—including ones involved in insulin-like signaling (ILS)—are at the core, indicating that GRN's structure is predictive of functionality. We used in vivo reporters and a novel functional network covering 5,497 genetic interactions to make mechanistic predictions. We used genetic epistasis to test some of these predictions, uncovering a novel transcriptional regulator, sup-37, that works alongside DAF-16/FOXO. We present a framework with predictive power that can accelerate discovery in C. elegans and potentially humans.
    Áreas temáticas: Multidisciplinary sciences Multidisciplinary
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: roger.guimera@urv.cat marta.sales@urv.cat
    Identificador del autor: 0000-0002-3597-4310 0000-0002-8140-6525
    Fecha de alta del registro: 2024-10-19
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.cell.com/iscience/fulltext/S2589-0042(21)01633-3?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2589004221016333%3Fshowall%3Dtrue
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Iscience. 25 (1): 103663-
    Referencia de l'ítem segons les normes APA: Suriyalaksh, Manusnan; Raimondi, Celia; Mains, Abraham; Segonds-Pichon, Anne; Mukhtar, Shahzabe; Murdoch, Sharlene; Aldunate, Rebeca; Krueger, Felix; (2022). Gene regulatory network inference in long-lived C. elegans reveals modular properties that are predictive of novel aging genes. Iscience, 25(1), 103663-. DOI: 10.1016/j.isci.2021.103663
    DOI del artículo: 10.1016/j.isci.2021.103663
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2022
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Multidisciplinary,Multidisciplinary Sciences
    Life-span
    Genomics
    Genetics
    Bioinformatics
    metabolism
    longevity
    insulin/igf-1
    identification
    expression
    evolution
    drosophila
    daf-16
    bow-ties
    Multidisciplinary sciences
    Multidisciplinary
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