Articles producció científica> Medicina i Cirurgia

Orally administered Odoribacter laneus improves glucose control and inflammatory profile in obese mice by depleting circulating succinate

  • Datos identificativos

    Identificador: imarina:9280939
    Autores:
    Huber-Ruano, IsabelCalvo, EnriqueMayneris-Perxachs, JordiRodriguez-Pena, M-MarCeperuelo-Mallafre, VictoriaCedo, LidiaNunez-Roa, CatalinaMiro-Blanch, JoanArnoriaga-Rodriguez, MariaBalvay, AurelieMaudet, ClaireGarcia-Roves, PabloYanes, OscarRabot, SylvieGrimaud, Ghjuvan MicaeluDe Prisco, AnnachiaraAmoruso, AngelaFernandez-Real, Jose ManuelVendrell, JoanFernandez-Veledo, Sonia
    Resumen:
    Background: Succinate is produced by both human cells and by gut bacteria and couples metabolism to inflammation as an extracellular signaling transducer. Circulating succinate is elevated in patients with obesity and type 2 diabetes and is linked to numerous complications, yet no studies have specifically addressed the contribution of gut microbiota to systemic succinate or explored the consequences of reducing intestinal succinate levels in this setting.Results: Using germ-free and microbiota-depleted mouse models, we show that the gut microbiota is a significant source of circulating succinate, which is elevated in obesity. We also show in vivo that therapeutic treatments with selected bacteria diminish the levels of circulating succinate in obese mice. Specifically, we demonstrate that Odoribacter laneus is a promising probiotic based on its ability to deplete succinate and improve glucose tolerance and the inflammatory profile in two independent models of obesity (db/db mice and diet-induced obese mice). Mechanistically, this is partly mediated by the succinate receptor 1. Supporting these preclinical findings, we demonstrate an inverse correlation between plasma and fecal levels of succinate in a cohort of patients with severe obesity. We also show that plasma succinate, which is associated with several components of metabolic syndrome including waist circumference, triglycerides, and uric acid, among others, is a primary determinant of insulin sensitivity evaluated by the euglycemic-hyperinsulinemic clamp.Conclusions: Overall, our work uncovers O. laneus as a promising next-generation probiotic to deplete succinate and improve glucose tolerance and obesity-related inflammation.
  • Otros:

    Autor según el artículo: Huber-Ruano, Isabel; Calvo, Enrique; Mayneris-Perxachs, Jordi; Rodriguez-Pena, M-Mar; Ceperuelo-Mallafre, Victoria; Cedo, Lidia; Nunez-Roa, Catalina; Miro-Blanch, Joan; Arnoriaga-Rodriguez, Maria; Balvay, Aurelie; Maudet, Claire; Garcia-Roves, Pablo; Yanes, Oscar; Rabot, Sylvie; Grimaud, Ghjuvan Micaelu; De Prisco, Annachiara; Amoruso, Angela; Fernandez-Real, Jose Manuel; Vendrell, Joan; Fernandez-Veledo, Sonia
    Departamento: Medicina i Cirurgia Eng. Electrònica, Elèctrica i Automàtica
    Autor/es de la URV: Ceperuelo Mallafré, Maria Victoria / Miró Blanch, Joan / Vendrell Ortega, Juan José / Yanes Torrado, Óscar
    Palabras clave: Triggers Succinate Sp nov. Signal Probiotics Obesity Microbiota Metabolism Inflammation Homeostasis Glucose tolerance Cells Animal models
    Resumen: Background: Succinate is produced by both human cells and by gut bacteria and couples metabolism to inflammation as an extracellular signaling transducer. Circulating succinate is elevated in patients with obesity and type 2 diabetes and is linked to numerous complications, yet no studies have specifically addressed the contribution of gut microbiota to systemic succinate or explored the consequences of reducing intestinal succinate levels in this setting.Results: Using germ-free and microbiota-depleted mouse models, we show that the gut microbiota is a significant source of circulating succinate, which is elevated in obesity. We also show in vivo that therapeutic treatments with selected bacteria diminish the levels of circulating succinate in obese mice. Specifically, we demonstrate that Odoribacter laneus is a promising probiotic based on its ability to deplete succinate and improve glucose tolerance and the inflammatory profile in two independent models of obesity (db/db mice and diet-induced obese mice). Mechanistically, this is partly mediated by the succinate receptor 1. Supporting these preclinical findings, we demonstrate an inverse correlation between plasma and fecal levels of succinate in a cohort of patients with severe obesity. We also show that plasma succinate, which is associated with several components of metabolic syndrome including waist circumference, triglycerides, and uric acid, among others, is a primary determinant of insulin sensitivity evaluated by the euglycemic-hyperinsulinemic clamp.Conclusions: Overall, our work uncovers O. laneus as a promising next-generation probiotic to deplete succinate and improve glucose tolerance and obesity-related inflammation.
    Áreas temáticas: Microbiology (medical) Microbiology Medicina i General medicine Ciências biológicas i
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: oscar.yanes@urv.cat victoria.ceperuelo@urv.cat joan.miro@estudiants.urv.cat juanjose.vendrell@urv.cat
    Identificador del autor: 0000-0003-3695-7157 0000-0002-4460-9761 0000-0003-3179-0554 0000-0002-6994-6115
    Fecha de alta del registro: 2024-10-12
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-022-01306-y
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Microbiome. 10 (135): 135-
    Referencia de l'ítem segons les normes APA: Huber-Ruano, Isabel; Calvo, Enrique; Mayneris-Perxachs, Jordi; Rodriguez-Pena, M-Mar; Ceperuelo-Mallafre, Victoria; Cedo, Lidia; Nunez-Roa, Catalina; (2022). Orally administered Odoribacter laneus improves glucose control and inflammatory profile in obese mice by depleting circulating succinate. Microbiome, 10(135), 135-. DOI: 10.1186/s40168-022-01306-y
    DOI del artículo: 10.1186/s40168-022-01306-y
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2022
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Microbiology,Microbiology (Medical)
    Triggers
    Succinate
    Sp nov.
    Signal
    Probiotics
    Obesity
    Microbiota
    Metabolism
    Inflammation
    Homeostasis
    Glucose tolerance
    Cells
    Animal models
    Microbiology (medical)
    Microbiology
    Medicina i
    General medicine
    Ciências biológicas i
  • Documentos:

  • Cerca a google

    Search to google scholar