Autor según el artículo: Manocchio F; Soliz-Rueda JR; Ribas-Latre A; Bravo FI; Arola-Arnal A; Suarez M; Muguerza B
Departamento: Bioquímica i Biotecnologia
Autor/es de la URV: Alkhoury, Nadine / Arola Arnal, Anna / Bravo Vázquez, Francisca Isabel / Manocchio, Francesca / Muguerza Marquínez, Maria Begoña / Ribas Latre, Aleix / Suárez Recio, Manuel
Palabras clave: Polyphenols Peripheral molecular clock Microrna Dose-dependent manner Cosinor-based rhythmometry Circadian rhythm rhythm rats polyphenols peripheral molecular clock mir-33a mir-122 microrna metabolism healthy expression cosinor-based rhythmometry bmal1 association
Resumen: Circadian rhythm is an endogenous and self-sustained timing system, responsible for the coordination of daily processes in 24-h timescale. It is regulated by an endogenous molecular clock, which is sensitive to external cues as light and food. We have previously shown that grape seed proanthocyanidins extract (GSPE) regulates the hepatic molecular clock. Moreover, GSPE is known to interact with some microRNAs (miRNAs). Therefore, the aim of this study was to evaluate if the activity of GSPE as modulator of hepatic clock genes could be mediated by miRNAs.250 mg/kg of GSPE was administered to Wistar rats before a 6-hour jet lag and sacrificed at different time points. GSPE modulated both expression of Bmal1 and miR-27b-3p in the liver. Cosinor-based analysis revealed that both Bmal1 and miR-27b-3p expression followed a circadian rhythm, a negative interaction between them, and the role of GSPE adjusting the hepatic peripheral clock via miRNA. Additionally, in vitro studies showed that Bmal1 was sensitive to GSPE (25 mg/L). However, this effect was independent of miR-27b-3p.miRNA regulation of peripheral clocks via GSPE may be part of a complex mechanism that involves the crosstalk with the central system rather than a direct effect. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.
Áreas temáticas: Saúde coletiva Química Nutrição Medicina veterinaria Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Educação física Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología Biotechnology Astronomia / física
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: nadine.alkhoury@estudiants.urv.cat nadine.alkhoury@estudiants.urv.cat nadine.alkhoury@estudiants.urv.cat nadine.alkhoury@estudiants.urv.cat francesca.manocchio@urv.cat francesca.manocchio@urv.cat francesca.manocchio@urv.cat manuel.suarez@urv.cat franciscaisabel.bravo@urv.cat anna.arola@urv.cat begona.muguerza@urv.cat
Identificador del autor: 0000-0003-0122-8253 0000-0002-6468-3088 0000-0001-6529-1345 0000-0001-7384-8588
Fecha de alta del registro: 2024-09-21
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://onlinelibrary.wiley.com/doi/full/10.1002/mnfr.202200443
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Molecular Nutrition & Food Research. 66 (23): e2200443-e2200443
Referencia de l'ítem segons les normes APA: Manocchio F; Soliz-Rueda JR; Ribas-Latre A; Bravo FI; Arola-Arnal A; Suarez M; Muguerza B (2022). Grape Seed Proanthocyanidins Modulate the Hepatic Molecular Clock via MicroRNAs. Molecular Nutrition & Food Research, 66(23), e2200443-e2200443. DOI: 10.1002/mnfr.202200443
DOI del artículo: 10.1002/mnfr.202200443
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2022
Tipo de publicación: Journal Publications