Autor según el artículo: Gunasekaran, Vijayalakshmi; Canela, Nuria; Constanti, Magda;
Departamento: Enginyeria Química
Autor/es de la URV: Constantí Garriga, Magdalena / GUNASEKARAN, VIJAYALAKSHMI
Palabras clave: Strain Sp nov. Pathway Mtbe Metaproteome Inhalation exposure Hydrocarbons Herbaspirillum-chlorophenolicum Etbe Degradation Biodegradation Bacterial consortium 2d-dige
Resumen: A bacterial consortium capable of degrading ethyl tert-butyl ether (ETBE) as a sole carbon source was enriched and isolated from gasoline-contaminated water. Arthrobacter sp., Herbaspirillum sp., Pseudacidovorax sp., Pseudomonas sp., and Xanthomonas sp. were identified as the initial populations with the 16S rDNA analysis. The consortium aerobically degraded 49% of 50 mg/L of ETBE, in 6 days. The ETBE degrading efficiency of the consortium increased to 98% even with the higher concentrations of ETBE (1000 mg/L) in the subsequent subcultures, which accumulated tert-butyl alcohol (TBA). Xanthomonas sp. and Pseudomonas sp. were identified as the predominant ETBE degrading populations in the final subculture. The metaproteome of the ETBE-grown bacterial consortium was compared with the glucose-grown bacterial consortium, using 2D-DIGE. Proteins related to the ETBE metabolism, stress response, carbon metabolism and chaperones were found to be abundant in the presence of ETBE while proteins related to cell division were less abundant. The metaproteomic study revealed that the ETBE does have an effect on the metabolism of the bacterial consortium. It also enabled us to understand the responses of the complex bacterial consortium to ETBE, thus revealing interesting facts about the ETBE degrading bacterial community.
Áreas temáticas: Virology Microbiology (medical) Microbiology
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: magdalena.constanti@urv.cat
Identificador del autor: 0000-0002-3547-4199
Fecha de alta del registro: 2024-09-07
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://www.mdpi.com/2076-2607/10/12/2331
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Microorganisms. 10 (12):
Referencia de l'ítem segons les normes APA: Gunasekaran, Vijayalakshmi; Canela, Nuria; Constanti, Magda; (2022). Comparative Proteomic Analysis of an Ethyl Tert-Butyl Ether-Degrading Bacterial Consortium. Microorganisms, 10(12), -. DOI: 10.3390/microorganisms10122331
DOI del artículo: 10.3390/microorganisms10122331
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2022
Tipo de publicación: Journal Publications