Autor según el artículo: Linklater, DP; Le Guével, X; Kosyer, E; Rubanov, S; Bryant, G; Hanssen, E; Baulin, VA; Pereiro, E; Perera, PG; Wandiyanto, JV; Angulo, A; Juodkazis, S; Ivanova, EP
Departamento: Química Física i Inorgànica
Autor/es de la URV: Baulin, Vladimir
Palabras clave: Toxicity Size Shape Nanotoxicity Nanoparticles Metal nanoclusters Ligand Gold nanoclusters
Resumen: Ultrasmall gold nanoclusters (AuNC) show great promise for application in theranostics due to their unique optical and physicochemical properties; however, the associated nanotoxicology concerns need to be carefully considered because of their high diffusion across the cellular barrier. Herein, new insights into the role of surface modification of 2 nm AuNC on their toxicity with impact on the metabolism of COS-7 fibroblast-like cells are revealed. AuNCs are chemically modified with either a monodentate-thiolated molecule (AuNC-MHA) or a modified-bidentate sulfobetaine zwitterionic molecule (AuNC-ZwBuEt). Uptake and localization inside fibroblasts and the resultant influence on cell ultrastructure are carefully evaluated using scanning transmission electron microscopy (STEM) and cryo-soft-X-ray tomography (cryo-SXT). At concentrations of >= 25 mu g Au mL(-1), AuNC-ZwBuEt are cytotoxic toward COS-7 cells and are observed to cross the nuclear membrane. Cryo-SXT analysis shows that fibroblasts develop an acute stress response in the form of swollen mitochondria, nuclear membrane blebbing, and large cytoplasmic vacuoles as early as 1 h postexposure. By contrast, AuNC-MHA are not cytotoxic toward COS-7 cells. Endosomal escape and translocation of the AuNC-ZwBuEt into the nucleus and other organelles may be the cause for the observed cytotoxicity and highlight the need for further study of metal nanocluster-cell interactions.
Áreas temáticas: Nanoscience & nanotechnology Medicine (miscellaneous) Materials science, biomaterials Engineering, biomedical Engineering (miscellaneous) Biomaterials Applied microbiology and biotechnology
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: vladimir.baulin@urv.cat
Identificador del autor: 0000-0003-2086-4271
Fecha de alta del registro: 2024-08-03
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://onlinelibrary.wiley.com/doi/full/10.1002/anbr.202200102
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Advanced Nanobiomed Research. 3 (4):
Referencia de l'ítem segons les normes APA: Linklater, DP; Le Guével, X; Kosyer, E; Rubanov, S; Bryant, G; Hanssen, E; Baulin, VA; Pereiro, E; Perera, PG; Wandiyanto, JV; Angulo, A; Juodkazis, S (2023). Functionalized Gold Nanoclusters Promote Stress Response in COS-7 Cells. Advanced Nanobiomed Research, 3(4), -. DOI: 10.1002/anbr.202200102
DOI del artículo: 10.1002/anbr.202200102
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2023
Tipo de publicación: Journal Publications