Autor según el artículo: Méndez, L; Muñoz, S; Barros, L; Miralles-Pérez, B; Romeu, M; Ramos-Romero, S; Torres, JL; Medina, I
Departamento: Ciències Mèdiques Bàsiques
Autor/es de la URV: Miralles Pérez, Bernat / Romeu Ferran, Marta
Palabras clave: Rats Oxidative stress Omega-3 pufas Marine omega-3 pufas Marine natural antioxidants Liver Lipid-peroxidation Kidney protein carbonylation Kidney lipotoxicity Ketamine Insulin-resistance Inflammation High-fat and high-sucrose diet Grape proanthocyanidins D-fagomine Combination
Resumen: Obesity has been recognized as a major risk factor for chronic kidney disease, insulin resistance being an early common metabolic feature in patients suffering from this syndrome. This study aims to investigate the mechanism underlying the induction of kidney dysfunction and the concomitant onset of insulin resistance by long-term high-fat and sucrose diet feeding in Sprague Dawley rats. To achieve this goal, our study analyzed renal carbonylated protein patterns, ectopic lipid accumulation and fatty acid profiles and correlated them with biometrical and biochemical measurements and other body redox status parameters. Rats fed the obesogenic diet developed a prediabetic state and incipient kidney dysfunction manifested in increased plasma urea concentration and superior levels of renal fat deposition and protein carbonylation. An obesogenic diet increased renal fat by preferentially promoting the accumulation of saturated fat, arachidonic, and docosahexaenoic fatty acids while decreasing oleic acid. Renal lipotoxicity was accompanied by selectively higher carbonylation of proteins involved in the blood pH regulation, i.e., bicarbonate reclamation and synthesis, amino acid, and glucose metabolisms, directly related to the onset of insulin resistance. This study also tested the combination of antioxidant properties of fish oil with the anti-diabetic properties of buckwheat D-Fagomine to counteract diet-induced renal alterations. Results demonstrated that bioactive compounds combined attenuated lipotoxicity, induced more favorable lipid profiles and counteracted the excessive carbonylation of proteins associated with pH regulation in the kidneys, resulting in an inhibition of the progression of the prediabetes state and kidney disease.
Áreas temáticas: Química Physiology Molecular biology Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Engenharias ii Clinical biochemistry Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry, medicinal Cell biology Biotecnología Biodiversidade Biochemistry & molecular biology Biochemistry
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: bernat.miralles@urv.cat marta.romeu@urv.cat
Identificador del autor: 0000-0003-1294-7069 0000-0002-2131-1858
Fecha de alta del registro: 2024-08-03
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://www.mdpi.com/2076-3921/12/3/751
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Antioxidants. 12 (3):
Referencia de l'ítem segons les normes APA: Méndez, L; Muñoz, S; Barros, L; Miralles-Pérez, B; Romeu, M; Ramos-Romero, S; Torres, JL; Medina, I (2023). Combined Intake of Fish Oil and D-Fagomine Prevents High-Fat High-Sucrose Diet-Induced Prediabetes by Modulating Lipotoxicity and Protein Carbonylation in the Kidney. Antioxidants, 12(3), -. DOI: 10.3390/antiox12030751
DOI del artículo: 10.3390/antiox12030751
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2023
Tipo de publicación: Journal Publications