Autor según el artículo: Granados-Casas, AO; Sastoque, AP; Stchigel, AM; Fernández-Bravo, A; Cano-Lira, JF
Departamento: Ciències Mèdiques Bàsiques
Autor/es de la URV: Cano Lira, José Francisco / Fernández Bravo, Ana / Granados Casas, Alan Omar / Sastoque Martínez, Angie Paola / Stchigel Glikman, Alberto Miguel
Palabras clave: Onygenales Genome Chrysosporium keratinophilum Biosynthetic pathways Ascomycota Ani onygenales genome chrysosporium keratinophilum biosynthetic pathways ascomycota
Resumen: Chrysosporium is a polyphyletic genus belonging (mostly) to different families of the order Onygenales (Eurotiomycetes, Ascomycota). Certain species, such as Chrysosporium keratinophilum, are pathogenic for animals, including humans, but are also a source of proteolytic enzymes (mainly keratinases) potentially useful in bioremediation. However, only a few studies have been published regarding bioactive compounds, of which the production is mostly unpredictable due to the absence of high-quality genomic sequences. During the development of our study, the genome of the ex-type strain of Chrysosporium keratinophilum, CBS 104.66, was sequenced and assembled using a hybrid method. The results showed a high-quality genome of 25.4 Mbp in size spread across 25 contigs, with an N50 of 2.0 Mb, 34,824 coding sequences, 8002 protein sequences, 166 tRNAs, and 24 rRNAs. The functional annotation of the predicted proteins was performed using InterProScan, and the KEGG pathway mapping using BlastKOALA. The results identified a total of 3529 protein families and 856 superfamilies, which were classified into six levels and 23 KEGG categories. Subsequently, using DIAMOND, we identified 83 pathogen–host interactions (PHI) and 421 carbohydrate-active enzymes (CAZymes). Finally, the analysis using AntiSMASH showed that this strain has a total of 27 biosynthesis gene clusters (BGCs), suggesting that it has a great potential to produce a wide variety of secondary metabolites. This genomic information provides new knowledge that allows for a deeper understanding of the biology of C. keratinophilum, and offers valuable new information for further investigations of the Chrysosporium species and the order Onygenales.
Áreas temáticas: Plant science Mycology Microbiology (medical) Microbiology Farmacia Ecology, evolution, behavior and systematics Ciências biológicas iii Ciências biológicas ii
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: angiepaola.sastoque@urv.cat ana.fernandez@urv.cat alanomar.granados@urv.cat jose.cano@urv.cat albertomiguel.stchigel@urv.cat angiepaola.sastoque@urv.cat alanomar.granados@urv.cat
Identificador del autor: 0000-0001-7637-5958 0000-0002-7339-7040 0000-0003-4495-4394 0000-0003-3987-7996 0000-0002-7339-7040
Fecha de alta del registro: 2024-01-13
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://www.mdpi.com/2309-608X/9/4/389
URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: J Fungi (Basel). 9 (4):
Referencia de l'ítem segons les normes APA: Granados-Casas, AO; Sastoque, AP; Stchigel, AM; Fernández-Bravo, A; Cano-Lira, JF (2023). Hybrid De Novo Whole-Genome Assembly, Annotation, and Identification of Secondary Metabolite Gene Clusters in the Ex-Type Strain of Chrysosporium keratinophilum. J Fungi (Basel), 9(4), -. DOI: 10.3390/jof9040389
DOI del artículo: 10.3390/jof9040389
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2023
Tipo de publicación: Journal Publications
Repositori URV