Articles producció científica> Ciències Mèdiques Bàsiques

Amesia hispanica sp. nov., Producer of the Antifungal Class of Antibiotics Dactylfungins

  • Datos identificativos

    Identificador: imarina:9296937
    Autores:
    Charria-Girón, EStchigel, AMCmoková, AKolarík, MSurup, FMarin-Felix, Y
    Resumen:
    During a study of the diversity of soilborne fungi from Spain, a strain belonging to the family Chaetomiaceae (Sordariales) was isolated. The multigene phylogenetic inference using five DNA loci showed that this strain represents an undescribed species of the genus Amesia, herein introduced as A. hispanica sp. nov. Investigation of its secondary metabolome led to the isolation of two new derivatives (2 and 3) of the known antifungal antibiotic dactylfungin A (1), together with the known compound cochliodinol (4). The planar structures of 1–4 were determined by ultrahigh performance liquid chromatography coupled with diode array detection and ion mobility tandem mass spectrometry (UHPLC-DAD-IM-MS/MS) and extensive 1D and 2D nuclear magnetic resonance (NMR) spectroscopy after isolation by HPLC. All isolated secondary metabolites were tested for their antimicrobial and cytotoxic activities. Dactylfungin A (1) showed selective and strong antifungal activity against some of the tested human pathogens (Aspergillus fumigatus and Cryptococcus neoformans). The additional hydroxyl group in 2 resulted in the loss of activity against C. neoformans but still retained the inhibition of As. fumigatus in a lower concentration than that of the respective control, without showing any cytotoxic effects. In contrast, 25″-dehydroxy-dactylfungin A (3) exhibited improved activity against yeasts (Schizosaccharomyces pombe and Rhodotorula glutinis) than 1 and 2, but resulted in the appearance of slight cytotoxicity. The present study exemplifies how even in a well-studied taxonomic group such as the Chaetomiaceae, the investigation of novel taxa still brings chemistry novelty, as demonstrated in this first report of this antibiotic class for chaetomiaceous and sordarialean taxa.
  • Otros:

    Autor según el artículo: Charria-Girón, E; Stchigel, AM; Cmoková, A; Kolarík, M; Surup, F; Marin-Felix, Y
    Departamento: Ciències Mèdiques Bàsiques
    Autor/es de la URV: MARÍN FÉLIX, YASMINA / Stchigel Glikman, Alberto Miguel
    Palabras clave: Sordariales Natural-products Metabolomics Fungal secondary metabolites Chaetomiaceae Antifungals sordariales resistance performance metabolomics metabolites fusarium fusapyrone fungus fungal secondary metabolites chaetomiaceae
    Resumen: During a study of the diversity of soilborne fungi from Spain, a strain belonging to the family Chaetomiaceae (Sordariales) was isolated. The multigene phylogenetic inference using five DNA loci showed that this strain represents an undescribed species of the genus Amesia, herein introduced as A. hispanica sp. nov. Investigation of its secondary metabolome led to the isolation of two new derivatives (2 and 3) of the known antifungal antibiotic dactylfungin A (1), together with the known compound cochliodinol (4). The planar structures of 1–4 were determined by ultrahigh performance liquid chromatography coupled with diode array detection and ion mobility tandem mass spectrometry (UHPLC-DAD-IM-MS/MS) and extensive 1D and 2D nuclear magnetic resonance (NMR) spectroscopy after isolation by HPLC. All isolated secondary metabolites were tested for their antimicrobial and cytotoxic activities. Dactylfungin A (1) showed selective and strong antifungal activity against some of the tested human pathogens (Aspergillus fumigatus and Cryptococcus neoformans). The additional hydroxyl group in 2 resulted in the loss of activity against C. neoformans but still retained the inhibition of As. fumigatus in a lower concentration than that of the respective control, without showing any cytotoxic effects. In contrast, 25″-dehydroxy-dactylfungin A (3) exhibited improved activity against yeasts (Schizosaccharomyces pombe and Rhodotorula glutinis) than 1 and 2, but resulted in the appearance of slight cytotoxicity. The present study exemplifies how even in a well-studied taxonomic group such as the Chaetomiaceae, the investigation of novel taxa still brings chemistry novelty, as demonstrated in this first report of this antibiotic class for chaetomiaceous and sordarialean taxa.
    Áreas temáticas: Plant science Mycology Microbiology (medical) Microbiology Farmacia Ecology, evolution, behavior and systematics Ciências biológicas iii Ciências biológicas ii
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: albertomiguel.stchigel@urv.cat
    Identificador del autor: 0000-0003-3987-7996
    Fecha de alta del registro: 2024-08-03
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.mdpi.com/2309-608X/9/4/463
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: J Fungi (Basel). 9 (4):
    Referencia de l'ítem segons les normes APA: Charria-Girón, E; Stchigel, AM; Cmoková, A; Kolarík, M; Surup, F; Marin-Felix, Y (2023). Amesia hispanica sp. nov., Producer of the Antifungal Class of Antibiotics Dactylfungins. J Fungi (Basel), 9(4), -. DOI: 10.3390/jof9040463
    DOI del artículo: 10.3390/jof9040463
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2023
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Ecology, Evolution, Behavior and Systematics,Microbiology,Microbiology (Medical),Mycology,Plant Science
    Sordariales
    Natural-products
    Metabolomics
    Fungal secondary metabolites
    Chaetomiaceae
    Antifungals
    sordariales
    resistance
    performance
    metabolomics
    metabolites
    fusarium
    fusapyrone
    fungus
    fungal secondary metabolites
    chaetomiaceae
    Plant science
    Mycology
    Microbiology (medical)
    Microbiology
    Farmacia
    Ecology, evolution, behavior and systematics
    Ciências biológicas iii
    Ciências biológicas ii
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