Articles producció científica> Medicina i Cirurgia

Lipidomics of triglyceride-rich lipoproteins derived from hyperlipidemic patients on inflammation

  • Datos identificativos

    Identificador: imarina:9332403
    Autores:
    Moreno-Vedia, JLlop, DRodríguez-Calvo, RPlana, NAmigó, NRosales, REsteban, YMasana, LIbarretxe, DGirona, J
    Resumen:
    Triglyceride-rich lipoproteins (TRLs) can have an important role in atherosclerosis development due to their size and ability to penetrate the endothelium. While high plasma triglyceride (TG) levels and chronic inflammation are relevant in metabolic diseases, it remains unclear whether TGs are atherogenic or which TRL-TG-derived metabolites are responsible for inflammation. Here, we aimed to study the lipidome modifications of TRL particles enriched in TG in patients with hyperlipidemia and their associations with a proinflammatory status both in vivo and in vitro.Using proton nuclear magnetic resonance (1 H-NMR), we analysed the plasma levels of glycoprotein acetyls and the TRL lipidomic profile of 307 patients with dyslipidemia. THP-1-derived macrophages were used as an in vitro model to explore the molecular inflammatory effects mediated by TRL.In vivo, higher TRL-TG levels were associated with higher circulating levels of NMR-measured glycoproteins (Glyc-A, Glyc-B and Glyc-F; p < .001). Lipidomic analysis showed that TRL-TG enrichment led to decreased cholesterol and phospholipid content (p < .01), an increase in omega-9, and a decrease in saturated fatty acids (p < .001). THP-1 macrophages exposed to increasing TRL particle concentrations augmented the secretion of IL-1β and TNF-α, which varied based on particle composition. Particles with higher cholesterol and phospholipid contents exerted higher cytokine secretion. The activation of MAPK, Akt/NFκB, and caspase-1 was concurrent with this proinflammatory response.High TRL-TG levels are associated with a higher systemic inflammatory status and increased particle concentrations. In vitro, higher particle numbers increase proinflammatory cytokine secretion, with cholesterol and phospholipid-rich TRL being more proinf
  • Otros:

    Autor según el artículo: Moreno-Vedia, J; Llop, D; Rodríguez-Calvo, R; Plana, N; Amigó, N; Rosales, R; Esteban, Y; Masana, L; Ibarretxe, D; Girona, J
    Departamento: Ciències Mèdiques Bàsiques Medicina i Cirurgia
    Autor/es de la URV: Girona Tell, Josefa / Ibarretxe Gerediaga, Daiana / Llop Paredes, Dídac / Masana Marín, Luis / Moreno Vedia, Juan / Plana Gil, Núria / Rodríguez Calvo, Ricardo / ROSALES RIBAS, ROSER
    Palabras clave: Triglyceride-rich lipoproteins Macrophages Inflammation H-1-nmr Atherosclerotic cardiovascular-disease Atherosclerosis 1h-nmr 1 h-nmr triglyceride-rich lipoproteins risk macrophages low-grade inflammation insights inflammation cholesterol atherosclerosis
    Resumen: Triglyceride-rich lipoproteins (TRLs) can have an important role in atherosclerosis development due to their size and ability to penetrate the endothelium. While high plasma triglyceride (TG) levels and chronic inflammation are relevant in metabolic diseases, it remains unclear whether TGs are atherogenic or which TRL-TG-derived metabolites are responsible for inflammation. Here, we aimed to study the lipidome modifications of TRL particles enriched in TG in patients with hyperlipidemia and their associations with a proinflammatory status both in vivo and in vitro.Using proton nuclear magnetic resonance (1 H-NMR), we analysed the plasma levels of glycoprotein acetyls and the TRL lipidomic profile of 307 patients with dyslipidemia. THP-1-derived macrophages were used as an in vitro model to explore the molecular inflammatory effects mediated by TRL.In vivo, higher TRL-TG levels were associated with higher circulating levels of NMR-measured glycoproteins (Glyc-A, Glyc-B and Glyc-F; p < .001). Lipidomic analysis showed that TRL-TG enrichment led to decreased cholesterol and phospholipid content (p < .01), an increase in omega-9, and a decrease in saturated fatty acids (p < .001). THP-1 macrophages exposed to increasing TRL particle concentrations augmented the secretion of IL-1β and TNF-α, which varied based on particle composition. Particles with higher cholesterol and phospholipid contents exerted higher cytokine secretion. The activation of MAPK, Akt/NFκB, and caspase-1 was concurrent with this proinflammatory response.High TRL-TG levels are associated with a higher systemic inflammatory status and increased particle concentrations. In vitro, higher particle numbers increase proinflammatory cytokine secretion, with cholesterol and phospholipid-rich TRL being more proinflammatory.© 2023 Stichting European Society for Clinical Investigation Journal Foundation. Published by John Wiley & Sons Ltd.
    Áreas temáticas: Saúde coletiva Odontología Nutrição Medicine, research & experimental Medicine, general & internal Medicine (miscellaneous) Medicina ii Medicina i Interdisciplinar General medicine Farmacia Educação física Clinical biochemistry Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Biotecnología Biochemistry
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: daiana.ibarretxe@urv.cat luis.masana@urv.cat didac.llop@urv.cat josefa.girona@urv.cat ricardo.rodriguez@urv.cat didac.llop@urv.cat josefa.girona@urv.cat juan.moreno@estudiants.urv.cat juan.moreno@estudiants.urv.cat
    Identificador del autor: 0000-0002-0789-4954 0000-0002-6267-8779 0000-0002-6267-8779 0000-0002-2673-3822 0000-0002-2673-3822
    Fecha de alta del registro: 2024-08-03
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://onlinelibrary.wiley.com/doi/epdf/10.1111/eci.14132
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: European Journal Of Clinical Investigation. 54 (3): e14132-e14132
    Referencia de l'ítem segons les normes APA: Moreno-Vedia, J; Llop, D; Rodríguez-Calvo, R; Plana, N; Amigó, N; Rosales, R; Esteban, Y; Masana, L; Ibarretxe, D; Girona, J (2024). Lipidomics of triglyceride-rich lipoproteins derived from hyperlipidemic patients on inflammation. European Journal Of Clinical Investigation, 54(3), e14132-e14132. DOI: 10.1111/eci.14132
    DOI del artículo: 10.1111/eci.14132
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2024
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Biochemistry,Clinical Biochemistry,Medicine (Miscellaneous),Medicine, General & Internal,Medicine, Research & Experimental
    Triglyceride-rich lipoproteins
    Macrophages
    Inflammation
    H-1-nmr
    Atherosclerotic cardiovascular-disease
    Atherosclerosis
    1h-nmr
    1 h-nmr
    triglyceride-rich lipoproteins
    risk
    macrophages
    low-grade inflammation
    insights
    inflammation
    cholesterol
    atherosclerosis
    Saúde coletiva
    Odontología
    Nutrição
    Medicine, research & experimental
    Medicine, general & internal
    Medicine (miscellaneous)
    Medicina ii
    Medicina i
    Interdisciplinar
    General medicine
    Farmacia
    Educação física
    Clinical biochemistry
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Biotecnología
    Biochemistry
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