Autor según el artículo: Hernandez-Baixauli, J; Chomiciute, G; Tracey, H; Mora, I; Cortés-Espinar, AJ; Avila-Román, J; Abasolo, N; Palacios-Jordan, H; Foguet-Romero, E; Suñol, D; Galofré, M; Alcaide-Hidalgo, JM; Baselga-Escudero, L; del Bas, JM; Mulero, M
Departamento: Bioquímica i Biotecnologia
Autor/es de la URV: Ávila Román, Francisco Javier / BASELGA ESCUDERO, LAURA
Palabras clave: Sphingomyelins Paraquat Oxidative stress Metabolism Lysophospholipids Gut microbiome Escherichia coli Akkermansia-muciniphila Akkermansia muciniphila 3-hydroxibutiric acid toxicity sphingomyelins peroxide oxidative stress metabolism lysophospholipids ketone-bodies inhibition health gut microbiome features exposure escherichia coli enzymes children akkermansia muciniphila 3-hydroxibutiric acid
Resumen: In this study, we examined the metabolic and gut microbiome responses to paraquat (PQ) in male Wistar rats, focusing on oxidative stress effects. Rats received a single intraperitoneal injection of PQ at 15 and 30 mg/kg, and various oxidative stress parameters (i.e., MDA, SOD, ROS, 8-isoprostanes) were assessed after three days. To explore the omic profile, GC-qTOF and UHPLC-qTOF were performed to assess the plasma metabolome; 1H-NMR was used to assess the urine metabolome; and shotgun metagenomics sequencing was performed to study the gut microbiome. Our results revealed reductions in body weight and tissue changes, particularly in the liver, were observed, suggesting a systemic effect of PQ. Elevated lipid peroxidation and reactive oxygen species levels in the liver and plasma indicated the induction of oxidative stress. Metabolic profiling revealed changes in the tricarboxylic acid cycle, accumulation of ketone body, and altered levels of key metabolites, such as 3-hydroxybutyric acid and serine, suggesting intricate links between energy metabolism and redox reactions. Plasma metabolomic analysis revealed alterations in mitochondrial metabolism, nicotinamide metabolism, and tryptophan degradation. The gut microbiome showed shifts, with higher PQ doses influencing microbial populations (e.g., Escherichia coli and Akkermansia muciniphila) and metagenomic functions (pyruvate metabolism, fermentation, nucleotide and amino acid biosynthesis). Overall, this study provides comprehensive insights into the complex interplay between PQ exposure, metabolic responses, and gut microbiome dynamics. These findings enhance our understanding of the mechanisms behind oxidative stress-induced metabolic alterations and underscore the connections between xenobiotic exposure, gut microbiota, and host metabolism.
Áreas temáticas: Química Physiology Molecular biology Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Engenharias ii Clinical biochemistry Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry, medicinal Cell biology Biotecnología Biodiversidade Biochemistry & molecular biology Biochemistry
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: franciscojavier.avila@urv.cat
Identificador del autor: 0000-0001-9766-8178
Fecha de alta del registro: 2024-02-17
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Referencia al articulo segun fuente origial: Antioxidants. 13 (1):
Referencia de l'ítem segons les normes APA: Hernandez-Baixauli, J; Chomiciute, G; Tracey, H; Mora, I; Cortés-Espinar, AJ; Avila-Román, J; Abasolo, N; Palacios-Jordan, H; Foguet-Romero, E; Suñol, (2024). Exploring Metabolic and Gut Microbiome Responses to Paraquat Administration in Male Wistar Rats: Implications for Oxidative Stress. Antioxidants, 13(1), -. DOI: 10.3390/antiox13010067
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2024
Tipo de publicación: Journal Publications
Repositori URV