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RT-qPCR dCT values of genes analyzed in colon, small intestine, liver and colon organoids

  • Identification data

    Identifier: PC:4131
    Authors:
    Canela, Nuria
    Abstract:
    RT-qPCR dCt values of gene expression from colon, small intestine and liver tissues from germ free mice monocolonized with E. coli expression bile salt hydrolase activity. Mice monocolonized with non-BSH expressing E. coli, non-monocolonized and conventionally-raised mice were used as controls, and RT-qpCR dCT values of colon organoids incubated with a mix of bile acids, LPS or a mix of bile acids + LPS. Values are normalized to the expression levels of ß-actin
  • Others:

    Subject matter: Bioquímica
    Access rights: info:eu-repo/semantics/openAccess
    Researcher identifier: 0000-0003-0261-2396
    Published by (editorial): Universitat Rovira i Virgili (URV)
    Related publications: Núñez-Sánchez, M. A., Herisson, F. M., Keane, J. M., García-González, N., Rossini, V., Pinhiero, J., Daly, J., Bustamante-Garrido, M., Hueston, C. M., Patel, S., Canela, N., Herrero, P., Claesson, M. J., Melgar, S., Nally, K., Caplice, N. M., & Gahan, C. G. M. (2022). Microbial bile salt hydrolase activity influences gene expression profiles and gastrointestinal maturation in infant mice. Gut Microbes, 14(1), 2149023. https://doi.org/10.1080/19490976.2022.2149023
    Abstract: RT-qPCR dCt values of gene expression from colon, small intestine and liver tissues from germ free mice monocolonized with E. coli expression bile salt hydrolase activity. Mice monocolonized with non-BSH expressing E. coli, non-monocolonized and conventionally-raised mice were used as controls, and RT-qpCR dCT values of colon organoids incubated with a mix of bile acids, LPS or a mix of bile acids + LPS. Values are normalized to the expression levels of ß-actin
    Departament: Bioquímica i Biotecnologia
    DOI: 10.5281/zenodo.6598104
    Document type: info:eu-repo/semantics/other
    Related publication's DOI: 10.1080/19490976.2022.2149023
    Repository ingest date: 2022-05-31
    Author: Canela, Nuria
    Keywords: Bile, organoid, colon, neonatal, infant, gut development, intestine, stem cell
    Dataset publication year: 2022
    Dataset title: RT-qPCR dCT values of genes analyzed in colon, small intestine, liver and colon organoids
  • Keywords:

    Bioquímica
    Bile, organoid, colon, neonatal, infant, gut development, intestine, stem cell
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