Blood-brain barrier monolayer model for transendothelial transport studies 

Identifier:  TFG:4352

Handle: https://hdl.handle.net/20.500.11797/TFG4352

Authors:  Llop Peiró, Ariadna

Abstract:
Due to the interest for PrPC protein and its transendothelial transport, it is necessary to develop in vitro blood-barrier models. In this study, we compare different endothelial cells, specifically bEnd.3 (mouse brain microvascular endothelial cells), and two human brain microvascular endothelial cells (HBMEC, from Cell Systems and Sciencell) in a monoculture model of the blood-brain barrier. We compare their barrier properties based on their transendothelial permeability and electrical resistance, as well as their morphology by immunocytochemical studies. This endothelial cell monolayer model is established in a transwell, which is a suitable system for transport studies. In the discussion of results, we conclude that HBMEC Sciencell cells are the most suitable cells for future experiments, due to their good results and morphology.