Immunohistochemical analysis of infection-induced long-term resident group 1 ILC populations in the skin

Identifier:  TFG:4453

Handle: https://hdl.handle.net/20.500.11797/TFG4453

Authors:  Visaconill Solé, Virgínia

Abstract:
Group 1 innate lymphoid cells (ILCs), which consist of Natural Killer (NK) cells and ILC1, contribute to the control of viral infections. We wanted to test whether NKs and ILC1s can also differentiate into tissue-resident cells in the adult organism during infection. Vaccinia virus (VV) was used to infect the ears skin of C57BL/6 mice and skin and lymph nodes were analysed by immunofluorescence analysis on day 3 and at week 5 post infection. Our analyses show that g1 ILCs are recruited to the skin upon VV infection and are able to persist long-term in the tissue, as well as local infection can dramatically alter the skin immune compartment and induce long-lasting changes in the tissue.