Putative partners for human aquaporin 4 in Saccharomyces cerevisiae

Identifier:  TFG:5018

Handle: https://hdl.handle.net/20.500.11797/TFG5018

Authors:  Ruiz Isant, Oriol

Abstract:
Aquaporin 4 (AQP4) is the most abundant aquaporin in the brain. Loss of AQP4 polarisation in the end-feet of astrocytes has been associated with many pathologies. It is believed that mislocalisation of AQP4 may happen due to protein-protein interactions. Therefore, this project focuses on screening a cDNA library from human brain mRNA to find new possible interaction partners of AQP4. To achieve this, bimolecular fluorescence complementation and fluorescence-activated cell sorting are used to achieve a higher-throughput screening. Two combinations have been tested, N-terminally tagged AQP4 or C-terminally tagged AQP4, combined with C-terminally tagged cDNA library. However, no positive hits have been found up to this date.