In vitro inmunomodulation of monocite cd14+ derived dendeitic cells with the antiviral drug ganciclovir

Identifier:  TFG:6609

Handle: https://hdl.handle.net/20.500.11797/TFG6609

Authors:  Romero Calvo, Daniel

Abstract:
DCs have a great influence on inflammation and the adaptive immune system. Previously, in mouse macrophages in vitro, GCV was seen to act as a partial agonist on the cGAS-STING pathway. This work studies whether it also occurs in human DCs. CD14+ monocytes were differentiated into DCs in vitro to study their activation with GCV. Analyzing them with FACS and RT-qPCR. Obtaining that GCV together with LPS, activator of cGAS-STING, enhances the activation. Increasing the expression of TNFa and IL-10, and increasing the abundance of CD80 and CD86. Confirming that GCV presents immunomodulatory capabilities on human DCs, enabling its use as an immunostimulator.