Creation date in repository: 2024-03-13
Abstract: Parkinson's Disease (PD) és a neurodegenerative dissorder characterized by degeneration dopaminergic neurons i accumulació protein aggregates in the brain. Aquest treball explora el rol de LRRK2 en l'endolysosomal pathway, investigant com el G2019S gain-of-function mutation disrupt vessic trafficking and protein degradation, contribuint al PD pathology. Additionally, els estudis investigats sobre l'involvement extracellular vehicles (EVs) in PD's inflammatory context, hypothesizing, que dysfunctional lysosomal clearance nucleic àcids leads per intracel·lular buildup, prompting both cellular inflammation and release. Experimental methods inclou EV isolació from WT i G2019S LRRK2 mutant cells, les seves característiques mitjançant Western blot analysis, i assessment de la inflamació signaling pathways mitjançant pattern recolliment receptor (PRR) lligands as stimulation. Les findings contribueixen a la nostra understanding de la interfície entre LRRK2 mutacions, lysosomal dysfunction, EVs, i inflamació en PD. Parkinson's Disease (PD) is a neurodegenerative disorder characterized by the degeneration of dopaminergic neurons and the accumulation of protein aggregates in the brain. This work explores the role of LRRK2 in the endolysosomal pathway, investigating how the G2019S gain-of-function mutation disrupt vesicle trafficking and protein degradation, contributing to PD pathology. Additionally, the study investigates the involvement of extracellular vesicles (EVs) in PD's inflammatory context, hypothesizing that dysfunctional lysosomal clearance of nucleic acids leads to intracellular buildup, prompting both cellular inflammation and the release of EVs. Experimental methods include EV isolation from WT and G2019S LRRK2 mutant cells, their characterization through Western blot analysis, and assessment of the inflammatory signalling pathways using pattern recognition receptor (PRR) ligands as stimulation. The findings contribute to our understanding of the interplay between LRRK2 mutations, lysosomal dysfunction, EVs, and inflammation in PD.
Subject: Biotecnologia
Language: en
Subject areas: Bioquímica i biotecnologia Biochemistry and biotechnology Bioquímica y biotecnología
Department: Bioquímica i Biotecnologia
Student: Rivas Galvez, Laia
Academic year: 2022-2023
Title in different languages: Estudi de l'efecte de la mutació G2019S en LRRK2: Comprenent la relació entre la inflamació perifèrica i la neurodegeneració a la Malaltia de Parkinson. Estudio del efecto de la mutación G2019S en LRRK2: Entendiendo la relación entre la inflamación periférica y la neurodegeneración en la Enfermedad de Parkinson.
Work's public defense date: 2023-09-14
Access rights: info:eu-repo/semantics/openAccess
Keywords: LRRK2, G2019S, vesícules extracel·lulars, cGAS-STING, Interferons de tipus 1, Parkinson LRRK2, G2019S, extracellular vesicles, cGAS-STING, Type I Interferon, Parkinson’s Disease LRRK2, G2019S, vesículas extracelulares, cGAS-STING, Interferones de tipo 1, Parkinson
Confidenciality: Si
Title in original language: Study of the effect of the G2019S mutation in LRRK2: Understanding the link between peripheral inflammation and neurodegeneration in Parkinson's Disease
Project director: Portillo Guisado, Maria del Carmen
Education area(s): Biotecnologia
Entity: Universitat Rovira i Virgili (URV)
Repositori URV